Cell Culture in the Study of Epithelial Development and Function
Certain cell lines derived from epithelial tissues have been demonstrated to retain in vitro some of the characteristics of their tissue of origin. Polarity of membrane proteins involved in transepithelial transport has been one of the most interesting characteristics that are preserved in these lines, thus allowing the study of ion and non-electrolyte transport in epithelia of simplified cellular composition. In addition they have become the model of choice in studies of the cell biology of membrane polarity generation.
KeywordsApical Membrane MDCK Cell Basolateral Membrane Confluent Monolayer Transepithelial Transport
Unable to display preview. Download preview PDF.
- Dharmsathaphorn, K. and Madera, J.L. (1989) Established intestinal cell lines as model systems for electrolyte transport studies. in: Methods in Enzymol: Biomembranes Biological Transport, ed. S. Fleisher and B. Fleisher. Orlando Florida: Academic Press (in press).Google Scholar
- Dharmsathaphorn, K., Mandel, K.G., Masui, H. and McRoberts, J.A. (1985) Vasoactive intestinal polypeptide-induced chloride secretion by a colonic epithelial cell line. Direct participation of a basolaterally localized Nay, Kam, Cl cotransport system. J. Clin. Invest., 75: 462–471.PubMedCrossRefGoogle Scholar
- Mandel, K.G., Dharmsathaphorn, K. andMcRoberts, J.A. (1986) Characterization of a cyclic AMP-activated Cl transport pathway in the apical membrane of a human colonic epithelial cell line. J. Biol. Chem., 261: 704–712.Google Scholar
- Rao, M.C. and Field, M. (1983) Role of calcium and cyclic nucleotides in the regulation of intestinal transport. in: Intestinal Transport, ed. Gilles-Baillien, M. and Gilles, K., pp. 228–239. Berlin: Springer Verlag.Google Scholar
- Rodriguez-Boulan, E., Vega-Salas, D., Sambuy, Y. and Salas, P.J.I. (1989) Mechanisms of segregation of apical and basolateral epithelial domains studied with enveloped viruses. in: Adaptation and Development of Gastrointestinal Function, ed. Smith, M.W. and Sepúlveda, F.V. pp. 115–130. Manchester: Manchester University Press.Google Scholar
- Zweibaum, A. and Chantret, I. (1989) Human colon carcinoma cell lines as in vitro models for the study of cell differentiation. in: Adaptation and Development of Gastrointestinal Function, ed. Smith, M.W. and Sepúlveda, F.V., pp 103–112. Manchester: Manchester University Press.Google Scholar