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Characterization of Transport Systems for Solutes at the Blood Side of Endothelial and Parenchymal Cells by Single Circulation Paired-Tracer Dilution: A Review of Recent Studies

  • David L. Yudilevich
  • Luis A. Sobrevía
  • L. Felipe Barros

Abstract

Carrier-mediated transport of blood-borne substrates at the blood-tissue interface in the microcirculation is a very rapid process and influx of labelled molecules can often be measured within the time of a single circulation through an organ. The plasma membranes involved in the tracer uptake may be those of (i) the endothelial cell, (ii) parenchymal cell and (iii) neuronal terminals.

Keywords

Amino Acid Transport Neutral Amino Acid Transport Amino Acid Transport System Single Circulation Reference Tracer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Barros, L.F., Bustamante, J.C., Yudilevich, D.L. and Jarvis, S.M. 1990. Adenosine transport and nitrobenzylthioinosine binding in human placental membrane vesicles from brush-border and basal sides of the trophoblast. J. Membr. Biol. 117, in press.Google Scholar
  2. Betz, A.L., Gilboe, D.D., Yudilevich, D.L. and Drewes, L.R. 1973. Kinetics of unidirectional glucose transport into the isolated dog brain. Am. J. Physiol. 225: 586–592.PubMedGoogle Scholar
  3. Betz, A.L., Gilboe, D.D. and Drewes, L.R. 1975. Kinetics of unidirectional leucine transport into brain: effects of isoleucine, valine, and anoxia. Am. J. Physiol. 228: 895–900.Google Scholar
  4. Boyd, C.A.R. and Yudilevich, D.L. 1987. Blood-tissue movement of amino acids in various organs: the basolateral membrane and its importance in the polarity of transepithelial transport. In: Amino Acid Transport in Animal Cells. Eds. D.L. Yudilevich and C.A.R. Boyd, pp. 120–156, Manchester University Press, ManchesterGoogle Scholar
  5. Bravo, I. and Sobrevía, L.. Kinetics of the L-alanine transport through the basolateral membrane in isolated oxyntic glands. Biochim. Biophys. Acta (in press).Google Scholar
  6. Bravo, I., Fuentes, O., Pozo, M. and Rojas, S. 1988. Kinetics of L-alanine and L-leucine transport at the blood-tissue interface in the perfused stomach. Cell. Mol. Biol. 34: 323–336.PubMedGoogle Scholar
  7. Brenton, D.P. and Gardiner, R.M. 1988. Transport of L-phenylalanine and related amino acids at the ovine blood-brain barrier. J. Physiol. 402: 497–508.Google Scholar
  8. Bustamante, J.C. and Setchell, B.P. 1982. Kinetics of the facilitated diffusion of leucine into the perfused testis of the rat. J. Physiol. 324: 49P.Google Scholar
  9. Bustamante, J.C., Mann, G.E. and Yudilevich, D.L. 1981. Specificity of amino acid uptake at the basolateral side of the epithelium in the cat salivary gland in situ. J.Physiol 313: 65–79Google Scholar
  10. Bustamante, J.C., Yudilevich, D.L. and Boyd, C.A.R. 1988. A new form of asymmetry in epithelia: Kinetics of apical and basal sulphate transport in human placenta. O. 7 Exper. Physiol. 73: 1013–1016Google Scholar
  11. Chaumet-Riffaud, Ph., Girault, M. and Syrota, A. (1984) Characterization of muscarinic cholinergic receptors in the isolated perfused rat heart. J. Physiol. 348: 11PGoogle Scholar
  12. Crone, C. 1963. The permeability of capillaries in various organs as determined by use of the indicator diffusion method’. Acta physiol. scand. 58: 292–305.Google Scholar
  13. Crone, C. 1965. Facilitated transfer of glucose from blood into brain tissue. J. Physiol. 181: 103–113.Google Scholar
  14. Christensen, H.N. 1990. Role of amino acid transport and countertransport in nutrition and metabolism. Physiol. Rev. 70: 43–76PubMedGoogle Scholar
  15. Christensen, H.N. 1982. Interorgan amino acid nutrition. Physiol. Rev. 62: 1193–1233Google Scholar
  16. Eaton, B.M. and Yudilevich, D.L. 1981. Uptake and asymmetric efflux of amino acids at maternal and fetal sides of placenta. Am. J. Physiol. 241: C106–112.Google Scholar
  17. Eaton, B.M., Mann, G.E. and Yudilevich, D.L. 1982. Transport specificity for neutral and basic amino acids at maternal and fetal interfaces of the guinea-pig placenta. J.Physiol. 328: 245–258.Google Scholar
  18. Haselton, F.R., Mueller, S.N., Howell, E.M., Levine, E.M. and Fishman, A.P. 1989. Chromatographic demonstration of reversible changes in endothelial permeability. J. Appl. Physiol. 67: 2032–2048.Google Scholar
  19. Hellewell, P.G. and Pearson, J.D. 1983 Kinetic analysis of prostaglandin F2 alpha and adenosine uptake in the piglet pulmonary circulation. J. Physiol. 343: 48P.Google Scholar
  20. Hundal, H.S., Rennie, M.J. and Watt, P.W. 1987. Characteristics of L-glutamine transport in perfused rat skeletal muscle. J. Physiol. 393: 283–305.PubMedGoogle Scholar
  21. Hundal, H.S., Rennie, M.J. and Watt, P.W. 1989. Characteristics of acidic, basic and neutral amino acid transport in the perfused rat hindlimb. J. Physiol. 408: 93–114.Google Scholar
  22. Mann, G.E. and Norman, P.S.R. 1984. Regulatory effects of insulin and experimental diabetes on neutral amino acid transport in the perfused rat exocrine pancreas: Kinetics of unidirectional L-serine influx and efflux at the basolateral plasma membrane. Biochim. Biophys. Acta 778: 618–622.Google Scholar
  23. Mann, G.E. and Peran, S. 1986. Basolateral amino acid transport systems in the perfused exocrine pancreas: sodium-dependency and kinetic interactions between influx and efflux mechanisms. Biochim. Biophys. Acta. 858: 263–274.PubMedCrossRefGoogle Scholar
  24. Mann, G.E. and Yudilevich, D.L. 1984a. Discrimination of parallel neutral amino acid transport systems in the basolateral membrane of cat salivary epithelium. J. Physiol. 347: 111127.Google Scholar
  25. Mann, G.E. and Yudilevich, D.L. 1984b. Rapid transcapillary exchange and unidirectional neuronal uptake of noradrenaline in the perfused rabbit heart. J. Physiol. 348: 589–600.Google Scholar
  26. Mann, G.E. and Yudilevich, D.L. 1987. Regulation of amino acid influx and efflux at the basolateral plasma membrane of the salivary epithelium: effects of parasympathetic nerve stimulation. J. Dental Res. 66: 569–575.CrossRefGoogle Scholar
  27. Mann, G.E., Pearson, J.D., Sheriff, C.-J. and Toothill, V.J. 1989. Expression of amino acid transport systems in cultured human umbilical vein endothelial cells. J. Physiol. 410: 325–339.Google Scholar
  28. Mann, G.E., Sheriff, C-J. and Pearson, J.D. 1990. N°-monomethyl-L-arginine and other cationic amino acids inhibit L-arginine uptake in perfused microcarrier cultures of bovine aortic endothelial cells. In: Nitric Oxide from L-Arginine: A Bioregulatory System, Eds. Moncada, S. and Higgs, E.A., pp. 331–339. Elsevier, Amsterdam.Google Scholar
  29. Mann, G.E., Wilson, S.M. and Yudilevich, D.L. 1984. Characteristics of a cationic amino acid transport system in the basolateral membrane of the cat salivary epithelium. J. Physiol. 351: 123–134.Google Scholar
  30. Mann, G.E., Zlokovic, B.V. and Yudilevich D.L. 1985. Evidence for a lactate transport system in the sarcolemmal membrane in the perfused rabbit heart: Kinetics of unidirectional influx, carrier specificity and effect of glucagon. Biochim. Biophys. Acta 819: 241248.Google Scholar
  31. Martin de Julian, P. and Yudilevich, D.L. 1964. A theory for the quantification of transcapillary exchange by tracer dilution curves. Am. J. Physiol. 207, 162–168.PubMedGoogle Scholar
  32. Mepham, T.B., Overthrow, J.I., Short, A.H., and Warne, D.E. 1984. Amino acid transport interactions in lactating mammary gland of guinea-pig. J. Physiol.357 73PGoogle Scholar
  33. Norman, P.S.R. 1988. Capillary extraction of [7,8 3H]dopamine in the isolated perfused rabbit heart. J. Physiol. 407: 16P.Google Scholar
  34. Norman, P.S.R. and Mann, G.E. 1986. Transport characteristics of System A in the rat exocrine pancreatic epithelium analyzed using the specific non-metabolized amino acid analogue ct-methylaminoisobutyric acid. Biochim. Biophys. Acta 861: 389–394.Google Scholar
  35. Norman, P.S.R. and Mann, G.E. 1987. Ionic dependence of amino-acid transport in the exocrine pancreatic epithelium: calcium dependence of insulin action. J. Membr. Biol. 96: 153163.Google Scholar
  36. Norman, P.S.R., Habara, Y. and Mann, G.E. 1989. Paradoxical effects of endogenous and exogenous insulin on amino acid transport activity in the isolated rat pancreas: somatostatin-14 inhibits insulin action. Diabetologia 32: 177–184.Google Scholar
  37. Perân, S. and McGee, M.P. 1986. Unidirectional flux of phenylalanine into Vero cells. Measurement using paired tracers in perfused cultures. Biochim. Biophys. Acta 856: 231–236.Google Scholar
  38. Perân, S., Munoz, M. and Saiz, M.T. 1990. Rapid and steady-state amino acid transport in perfused human fibroblasts and colon adenocarcinoma cells: effects of methotrexate. Biochim. Biophys. Acta 1024: 233–240.Google Scholar
  39. Rennie, M.J., Idstrom, J-P., Mann, G.E., Schersten, T. and Bylund-Fellinius, A-C. 1983. A paired-tracer dilution method for characterizing membrane transport in the perfused rat hindlimb. Biochem. J. 214: 737–743.Google Scholar
  40. Smith, I.C.H. 1985. An interpretation of single-pass uptake curves for measuring cellular transport. In: Carrier-Mediated Transport of Solutes from Blood to Tissue. Eds. Yudilevich, D.L. and Mann, G.E., pp. 397–402. Longman, London.Google Scholar
  41. Sweiry, J.H. and Yudilevich, D.L. 1984. Asymmetric calcium influx and efflux at maternal and fetal sides of the guinea-pig placenta: kinetics and specificity. J. Physiol. 355: 295–311.Google Scholar
  42. Sweiry, J.H. and Yudilevich, D.L. 1985. Characterization of choline transport at maternal and fetal interfaces of the perfused guinea-pig placenta. J. Physiol. 366: 251–266.Google Scholar
  43. Sweiry, J.H. and Yudilevich, D.L. 1988. Characterization of folate uptake in guinea pig placenta. Am. J. Physiol. 254: C735–C743.PubMedGoogle Scholar
  44. Sweiry, J.H., Page, K.R., Dacke, C.G., Abramovich, D.R. and Yudilevich, D.L. 1986. Evidence of saturable uptake mechanisms at maternal and fetal sides of the perfused human placenta by rapid paired-tracer dilution: studies with calcium and choline. J. Dev. Physiol. 8: 435–445Google Scholar
  45. Syrota, A., Girault, M., Pocidalo, J-J. and Yudilevich, D.L. 1982. Endothelial uptake of amino acids, sugars, lipids, and prostaglandins in rat lung. Am. J. Physiol. 243: C20–C26.Google Scholar
  46. Taylor, P.M. and Rennie, M.J. 1987. Perivenous localisation of Na-dependent glutamate transport in perfused rat liver. FEBS Lett 221: 370–374.Google Scholar
  47. Taylor, P.M. and Rennie, M.J. 1988. Amino acid fluxes across sinusoidal membranes of the perfused rat liver: relationships with portal ammonia concentration. In: Advances in Ammonia Metabolism and Hepatic Encephalopathy. Eds. Soelers, P.B., Wilson, J.H.P, Meyer, A.J., Holm, E. Amsterdam: Elsevier, pp, 45–52.Google Scholar
  48. Taylor, P.M., Egan, C.J. and Rennie, M.J. 1989. Transport of glutamine across blood-facing membranes of perfused rat jejunum. Am. J. Physiol. 256: E550–E558.Google Scholar
  49. Toothill, V.J., Eaton, B.M., Davis, H., Pearson, J.D. and Mann, G.E. Solute permeability of endothelial cell junctions in perfused microcarrier cultures of human umbilical vein endothelial cells. Int. J. Microcir. Clin. Exp. (abstract in press).Google Scholar
  50. Wangler, R.D., Gorman, M.W., Wang, C.Y., DeWitt, D.F., Chan, I.S., Bassingthwaighte, J.B. and Sparks, H.V. 1989. Transcapillary adenosine transport and interstitial adenosine concentration in guinea pig hearts. Am. J Physiol. 257: H89–106.PubMedGoogle Scholar
  51. Watt, P.W., MacLennan, P.A., Hundal, H.S., Kuret, C.M. and Rennie, M.J. 1988. L(+)-Lactate transport in perfused rat skeletal muscle: kinetic characteristics and sensitivity to pH and transport inhibitors. Biochim. Biophys. Acta 944: 213–222.Google Scholar
  52. Wheeler, C.P.D. and Yudilevich, D.L. 1988. Transport and metabolism of adenosine in the perfused guinea-pig placenta. J. Physiol. 405: 511–526.Google Scholar
  53. Wheeler, C.P.D. and Yudilevich, D.L. 1989a. Effect of insulin, prostaglandin El and uptake inhibitors on glucose transport in the perfused guinea-pig placenta. J. Dev. Physiol. 11: 159–169.Google Scholar
  54. Wheeler, C.P.D. and Yudilevich, D.L. 1989b. Lysine and alanine transport in the perfused guinea-pig placenta. Biochim. Biophys. Acta 978: 257–266.Google Scholar
  55. Yudilevich, D.L. 1985. Characterisation of membrane carriers and receptors at the blood-tissue interface by single circulation paired-tracer dilution. In: Carrier mediated transport of solutes from Blood to Tissue. Eds. Yudilevich, D.L. and Mann, G.E., pp. 1–31. Longman, London.Google Scholar
  56. Yudilevich, D.L. 1989. Blood-tissue transport of substrates in the heart: studies by single circulation tracer dilution. Int. J. Microcirc. Clin.Exp. 8: 397–409.Google Scholar
  57. Yudilevich, D.L. and Barros, L.F. 1990. Transport of amino acids and nucleosides in the placenta. Biochem. Soc. Transactions 14: 1146–1150Google Scholar
  58. Yudilevich, D.L. and Eaton, B.M. 1980. Amino acid carriers at the maternal and fetal surfaces of placenta by single circulation paired-tracer dilution. Kinetics of phenylalanine transport. Biochim. Biophys. Acta 596: 315–319PubMedCrossRefGoogle Scholar
  59. Yudilevich, D.L. and Mann, G.E. 1982. Unidirectional uptake of substrates at the blood-side of secretory epithelia: stomach, salivary gland, pancreas. Fed. Proc. 41: 3045–3053.Google Scholar
  60. Yudilevich, D.L. and Mann, G.E. (eds.) 1985. Carrier-mediated Transport of Solutes from Blood to Tissue. Longman, London.Google Scholar
  61. Yudilevich, D.L. and Sweiry, J.H. 1985. Transport of amino acids in the placenta. Biochim. Biophys. Acta 822: 169–201.Google Scholar
  62. Yudilevich, D.L. and Wheeler, C.D.P. 1988. Placental transport: the brush border and the basal sides of the trophoblast, and a role for the fetal endothelium?. In: Fetal and Neonatal Development. Ed. Jones, C.T., pp. 47–57. Perinatology Press, New York.Google Scholar
  63. Yudilevich, D.L., de Rose, N. and Sepulveda, F.V. 1972. Facilitated transport of amino acid through the blood-brain barrier of the dog studied in a single capillary circulation. Brain Res 44: 569–578.Google Scholar
  64. Yudilevich, D.L., Eaton, B.M., Short, A.H. and Leichtweiss, H.-P. 1979. Glucose carriers at maternal and fetal sides of the trophoblast in guinea-pig placenta. Am. J. Physiol. C205–212.Google Scholar
  65. Zlokovic, B.V., Segal, M.B., Begley, D.J., Dayson, H. and Rakic, L. 1985. Permeability of the blood-cerebrospinal fluid and blood-brain barriers to thyrotropin-releasing hormone. Brain Research 358: 191–199.Google Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • David L. Yudilevich
    • 1
    • 2
  • Luis A. Sobrevía
    • 1
    • 2
  • L. Felipe Barros
    • 1
    • 2
  1. 1.Physiology, Biomedical Sciences Division, King’s CollegeUniversity of LondonLondonUK
  2. 2.Departamento de Fisiología y Biofísica, Facultad de MedicinaUniversidad de ChileSantiagoChile

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