Single-Channel and Whole-Cell Patch-Clamp Experiments on Gland Cells: Activation of Ion Channels Via Internal Messengers
Electrophysiological studies on gland cells have contributed much to our understanding of the various membrane transport events and the stimulus-secretion coupling sequences. The earliest microelectrode studies on exocrine glands were carried out by Lundberg (1958) and later extended by Petersen (1980). The small size of mammalian gland cells made proper voltage clamp studies of transmembrane ionic currents difficult, but the first such studies with intracellular microelectrodes were made on pancreatic acini by McCandless et al. (1981) and Maruyama & Petersen (1983). At this time, however, the improved patch-clamp technique (Hamill et al. (1981) had become available and the first studies using this technique on exocrine glands were made by Maruyama and Petersen (1982a,b) on membrane fragments and intact single pancreatic acinar cells. This review aims to explain how the various patch-clamp recording configurations have been used to investigate the mechanisms by which receptor-activation switches on ion channels in exocrine gland cells.
KeywordsAcinar Cell Pancreatic Acinar Cell Pancreatic Acinus Inositol Trisphosphate Mouse Pancreatic Acinar Cell
Unable to display preview. Download preview PDF.
- 10.Lundberg, A., 1958, Electrophysiology of salivary glands, Physiol. Rev., 38: 2140.Google Scholar
- 15.Maruyama, Y. and Petersen, O.H., 1982b, Cholecystokinin activation of single–channel currents is mediated by internal messenger in pancreatic acinar cells, Nature, 300–61–63.Google Scholar
- 22.Osipchuk, Y.V., Wakui, M., Yule, D.I., Gallacher, D.V. and Petersen, O.H., 1990, Cytoplasmic Ca2+ oscillations evoked by receptor stimulation, G-protein activation, internal application of inositol trisphosphate or Ca2+: simultaneous microfluorimetry and Ca2+-dependent C1’ current recording in single pancreatic acinar cells, EMBO J., 9: 697–704PubMedGoogle Scholar
- 23.Petersen, O.H., 1980, The Electrophysiology of Gland Cells, Academic Press: New York.Google Scholar
- 27.Petersen, O.H. and Wakui, M., 1990, Oscillating intracellular Cat+ signals evoked by activation of receptors linked to inositol lipid hydrolysis: mechanism of generation, J. Membr. Biol., in the press.Google Scholar