Methodological Guide for Studying Epithelial Transport with Isolated Membrane Vesicles
Conceptually, studies with isolated plasma membranes can be placed between traditional physiological approaches and traditional biochemical approaches. In the former, the living cell is regarded as the minimum entity in which processes underlying the function of cells can be investigated; in the latter, cellular components usually have to be purified to homogeneity before studies on the properties of the component can be initiated. Thus studies on isolated membranes can focus either on questions related to cellular physiology, on questions related to the molecular properties of membrane components, or on both.
KeywordsMembrane Vesicle Brush Border Membrane Kidney Cortex Brush Border Membrane Vesicle Membrane Biol
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