Cell Fusion pp 441-456 | Cite as

Micromanipulation and Elastic Response of Electrically Fused Red Cells

  • Donna M. Miles
  • Robert M. Hochmuth


Fusion of human erythrocytes may be obtained by several methods such as incubation with uranyl ions or some rare earth metals (Majumdar etal., 1980), treatment with calcium and phosphate ions (Baker and Kalra, 1979), incubation with viral fusogens such as the Sendai virus (Knutton, 19796), and treatment with polyethylene glycol (Knutton, 1979a). The method of electrofusion is relatively new in comparison and in its early stages of investigation and application. According to Zimmermann and Vienken (1982), electrofusion has several advantages compared with other methods of fusion: (1) the number of cells to be fused may be selected, (2) the loss of intracellular contents is decreased, and (3) the membrane properties are not changed by the addition of other substances.


Alternate Current Direct Current Pulse Aspiration Pressure Cell Doublet Alternate Current Field 
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  1. Baker, R. F., and Kalra, V. K., 1979, Chemically induced fusion of human erythrocytes, Biochem. Biophys. Res. Commun. 86:920–928.PubMedCrossRefGoogle Scholar
  2. Benz, R., Beckers, F., and Zimmermann, U., 1979, Reversible electrical breakdown of lipid bilayer membranes: A charge-pulse relaxation study, J. Membrane Biol. 48:181–204.CrossRefGoogle Scholar
  3. Donlon, J. A., and Rothstein, A., 1969, The cation permeability of erythrocytes in low ionic strength media of various tonicities, J. Membrane Biol. 1:37–52.CrossRefGoogle Scholar
  4. Evans, E. A., 1973, A new material concept for the red cell membrane, Biophys. J. 13:926–939.PubMedCrossRefGoogle Scholar
  5. Evans, E. A., 1983, Bending elastic modulus of red blood cell membrane derived from buckling instability in micropipet aspiration tests, Biophys. J. 43:27–30.PubMedCrossRefGoogle Scholar
  6. Evans, E. A., 1985, Detailed mechanics of membrane-membrane adhesion and separation. I. Continuum of molecular cross-bridges, Biophys. J. 48:175–183.PubMedCrossRefGoogle Scholar
  7. Evans, E. A., Waugh, R., and Melnik, L., 1976, Elastic area compressibility modulus of red cell membrane, Biophys. J. 16:585–595.PubMedCrossRefGoogle Scholar
  8. Kinosita, K., Jr., and Tsong, T. Y., 1977, Voltage-induced pore formation and hemolysis of human erythrocytes, Biochim. Biophys. Acta 471:227–242.PubMedCrossRefGoogle Scholar
  9. Knutton, S., 1979a, Studies of membrane fusion. III. Fusion of erythrocytes with polyethylene glycol, J. Cell Sci. 36:61–72.PubMedGoogle Scholar
  10. Knutton, S., 1979, Studies of membrane fusion. V. Fusion of erythrocytes with non-haemolytic Sendai virus, J. Cell Sci. 36:85–96.PubMedGoogle Scholar
  11. Linderkamp, O., and Meiselman, H. J., 1982, Geometric, osmotic, and membrane mechanical properties of density-separated human red cells, Blood 59:1121–1127.PubMedGoogle Scholar
  12. Majumdar, S., Baker, R. F., and Kalra, V. K., 1980, Fusion of human erythrocytes induced by uranyl acetate and rare earth metals, Biochim. Biophys. Acta 598:411–416.PubMedCrossRefGoogle Scholar
  13. Nash, G. B., Johnson, C. S., and Meiselman, H. J., 1984, Mechanical properties of oxygenated red blood cells in sickle cell disease, Blood 63:73–82.PubMedGoogle Scholar
  14. Pohl, H. A., 1978, Dielectrophoresis, Cambridge University Press, Cambridge.Google Scholar
  15. Rand, R. P., 1981, Interacting phospholipid bilayers: Measured forces and induced structural changes, Annu. Rev. Biophys. Bioeng. 10:277–314.PubMedCrossRefGoogle Scholar
  16. Riemann, F., Zimmermann, U., and Pilwat, G., 1975, Release and uptake of haemoglobin and ions in red blood cells induced by dielectric breakdown, Biochim. Biophys. Acta 394:449–462.PubMedCrossRefGoogle Scholar
  17. Sale, A. J. H., and Hamilton, W. A., 1968, Effects of high electric fields on micro-organisms. III. Lysis of erythrocytes and protoplasts, Biochim. Biophys. Acta 163:37–43.PubMedCrossRefGoogle Scholar
  18. Sowers, A. E., 1984, Characterization of electric field-induced fusion in erythrocyte ghost membranes, J. Cell Biol. 99:1989–1996.PubMedCrossRefGoogle Scholar
  19. Takashima, S., and Schwan, H. P., 1985, Alignment of microscopic particles in electric fields and its biological implications, Biophys. J. 47:513–518.PubMedCrossRefGoogle Scholar
  20. Waugh, R., and Evans, E. A., 1979, Thermoelasticity of red blood cell membrane, Biophys. J. 26:115–132.PubMedCrossRefGoogle Scholar
  21. Zimmermann, U., 1982, Electric field-mediated fusion and related electrical phenomena, Biochim. Biophys. Acta 694:227–277.PubMedCrossRefGoogle Scholar
  22. Zimmermann, U., and Vienken, J., 1982, Electric field-induced cell-to-cell fusion, J. Membrane Biol. 67:165–182.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Donna M. Miles
    • 1
  • Robert M. Hochmuth
    • 1
  1. 1.Department of Biomedical EngineeringDuke UniversityDurhamUSA

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