Cell Fusion pp 409-418 | Cite as

Laser-Induced Cell Fusion

  • Einhard Schierenberg


In recent years a number of different techniques have been developed to fuse cells of various origin in order to obtain hybrids with qualities different from each of the original cells. In many cases, one wants to fuse large numbers of cells (e.g., to generate hybridomas), and fusion occurs more or less randomly (e.g., Köhler and Milstein, 1975). With other methods, individual cells have been fused under controlled conditions (e.g., McGrath and Solter, 1984). So far a prerequisite for fusing two preselected cells has been that they must be isolated away from other cells.


Cell Fusion Nematode Caenorhabditis Elegans Laser Microbeam Fast Repetition Rate Broad Contact 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bereiter-Hahn, J., 1972, Laser micro-radiation as a tool in biology and medicine. Part 2: Method and Applications, Microsc. Acta 72:1–33.PubMedGoogle Scholar
  2. Berns, M. W., 1972, Partial cell irradiation with a tuneable organic dye laser, Nature (Lond.) 240:483–484.CrossRefGoogle Scholar
  3. Berns, M. W., 1976, Biological Microirradiation, Prentice-Hall, Englewood Cliffs, New Jersey.Google Scholar
  4. Boveri, T., 1899, Die Entwicklung von Ascaris megalocephala mit besonderer Rücksicht auf die Kernverhältnisse, Festschrift für C. v. Kupffer, Fischer, Jena, pp. 383–430.CrossRefGoogle Scholar
  5. Chitwood, B. G., and Chitwood, M. B., 1974, Introduction to NematologY., University Park Press, Baltimore.Google Scholar
  6. Davidson, E. H., 1976, Gene Activity in Early Development, 2nd ed., Academic Press, New York.Google Scholar
  7. Deppe, U., Schierenberg, E., Cole, T., Krieg, C., Schmitt, D., Yoder, B., and von Ehrenstein, G., 1978, Cell lineages of the embryo of the nematode Caenorhabditis elegans, Proc. Natl Acad. Sci. U.S.A. 75:376–380.CrossRefGoogle Scholar
  8. Hochstrasser, R. M., and Johnson, C. K., 1985, Lasers in biology, Laser Focus/ElectroOpt. 21:100–118.Google Scholar
  9. Isenberg, G., Bielser, W., Meier-Ruge, W., and Remy, E., 1976, Cell surgery by laser microdissection: a preparative method, J. Microsc. 107:19–24.PubMedCrossRefGoogle Scholar
  10. Jacobson, K., Elson, E., Koppel, E., and Webb, W., 1983, International workshop on the application of fluorescence photobleaching techniques to problems in cell biology., Fed. Proc. 42:72–79.PubMedGoogle Scholar
  11. Laufer, J. S., and von Ehrenstein, G., 1981, Nematode development after removal of egg cytoplasm: Absence of localized unbound determinants, Science 211:402–405.PubMedCrossRefGoogle Scholar
  12. Kimble, J. E., and White, J. G., 1981, On the control of germ cell development in Caenorhabditis elegans, Dev. Biol. 81:208–219.CrossRefGoogle Scholar
  13. Köhler, G., and Milstein, C., 1975, Continuous culture of fused cells secreting antibody of predefined specificity, Nature (Lond.) 256:495–497.CrossRefGoogle Scholar
  14. McGrath, J., and Solter, D., 1984, Nuclear transplantation in the mouse embryo by microsurgery and cell fusion, Science 220:1300–1302.CrossRefGoogle Scholar
  15. Meier-Ruge, W., Bielser, W., Remy, E., Hillenkamp, F., Nitsche, R., and Unsöld, E., 1976, The laser in the Lowry technique for microdissection of freeze-dried tissue slices, Histochem. J. 8:387–401.PubMedCrossRefGoogle Scholar
  16. Moreno, G., Lutz, M., and Bessis, M., 1969, Partial cell irradiation by ultraviolet and visible light: conventional and laser sources, Int. Rev. Exp. Pathol. 7:99–137.PubMedGoogle Scholar
  17. Pataki, A., Meier-Ruge, W., Wiederhold, K. H., and Remy, E., 1978, The laser micro beam as a tool for tissue sampling in biochemistry, Laser ElektroOptik 1:14–16.Google Scholar
  18. Salmon, E. D., Saxton, W. M., Leslie, R. J., Karow, M. L., and Mcintosh, J. R., 1984, Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin: Video image analysis of fluorescence redistribution after photobleaching, J. Cell Biol. 99:2157–2164.PubMedCrossRefGoogle Scholar
  19. Schierenberg, E., 1982, Development of the nematode Caenorhabditis elegans, in: Developmental Biology of Freshwater Invertebrates (F. W. Harrison and R. R. Cowden, eds.), pp. 249–281, Liss, New York.Google Scholar
  20. Schierenberg, E., 1984, Altered cell-division rates after laser-induced cell fusion in nematode embryos, Dev. Biol. 101:240–245.PubMedCrossRefGoogle Scholar
  21. Schierenberg, E., 1985, Cell determination during early embryogenesis of the nematode Caenorhabditis elegans, Cold Spring Harbor Symp. Quant. Biol. 50:59–68.CrossRefGoogle Scholar
  22. Schierenberg, E., and Wood, W. B., 1985, Control of cell-cycle timing in early embryos of Caenorhabditis elegans, Dev. Biol. 107:337–354.PubMedCrossRefGoogle Scholar
  23. Schierenberg, E., Miwa, J., and von Ehrenstein, G., 1980, Cell lineages and developmental defects of temperature-sensitive embryonic arrest mutants in Caenorhabditis elegans, Dev. Biol. 76:141–159.CrossRefGoogle Scholar
  24. Sulston, J. E., and Horvitz, H. R., 1977, Post-embryonic cell lineages of the nematode Caenorhabditis elegans, Dev. Biol. 56:110–156.CrossRefGoogle Scholar
  25. Sulston, J. E., Schierenberg, E., White, J., and Thomson, N., 1983, The embryonic cell lineage of the nematode Carnorhabditis elegans, Dev. Biol. 100:64–119.CrossRefGoogle Scholar
  26. White, J. G., and Horvitz, H. R., 1979, Laser microbeam techniques in biological research, Electro-Opt. Syst. Design August:23–24.Google Scholar
  27. Wood, W. B., Schierenberg, E., and Strome, S., 1984, Localization and determination in early embryos of Caenorhabditis elegans, in: Molecular Biology of Development (E. H. Davidson and R. A. Firtel, eds.), pp. 37–49, Liss., New York.Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Einhard Schierenberg
    • 1
  1. 1.Abteilung ChemieMax-Planck-Institut für Experimentelle MedizinGöttingenFederal Republic of Germany

Personalised recommendations