Ion Microscopy Imaging of Boron from BNCT Drugs in Cryogenically Prepared Tissues
Histopathologically prepared sections from brain tissue of patients with diseases such as glioblastoma multiforme can reveal satellite regions which are small clusters of malignant cells adjacent to a main tumor mass but embedded in the contiguous healthy brain tissue. Within the BNCT community, as well as the rest of the medical community, it is a well known fact that many of these small tumor satellites cannot be removed during the surgical debulking procedures used to eliminate the main tumor mass. Additionally, the small tumor satellites which remain after surgical treatment are responsible for the regrowth and recurrence of the associated disease. If compounds such as p-boronophenylalanine (BPA) or sodium mercaptoundecahydrododecaborate (BSH) are going to be used successfully in BNCT, then they must selectively target the smallest possible clusters of malignant tumor cells. Thus, it is crucial to provide direct evidence of the selective delivery of boron at the cellular and subcellular level prior to acceptance of these compounds as clinical agents for BNCT. Although analytical techniques such as quantitative neutron capture radiography1 are highly sensitive, they lack the spatial resolution required for analyzing the subcellular distribution of boron. In contrast, ion microscopy is capable of resolving subcellular features such as nucleus, cytoplasm, etc. with a spatial resolution of 0.5 μm.
KeywordsBrush Border Boron Neutron Capture Therapy Optical Microscopy Image Proximal Convoluted Tubule Interlobular Artery
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