Penner Serotyping and Polymerase Chain Reaction Fingerprinting of Campylobacter Isolated from Poultry and Other Animal Sources
Foods of animal origin, and in particular poultry products, are recognized as the most important vehicles of Campylobacter infection in humans. Reduction of Campylobacter contamination of poultry could therefore reduce the risk of campylobacteriosis to consumers. However, the epidemiology of Campylobacter colonization in poultry is not completely understood. Horizontal transmission via the farm environment seems to be the major route for Campylobacter contamination of poultry4. Whereas, vertical transmission of Campylobacter via the egg, from positive breeder flocks to their progeny2,8,9 and horizontal transmission of Campylobacter from one broiler flock to the next one via a persistent contamination within the broiler house4 has not been found to be very likely. Serotyping is often used to support epidemiological investigations. However, serotyping has the disadvantage of having low discriminatory power and additionally, a substantial number of Campylobacter strains are nontypable. Therefore, we have used PCR fingerprinting in this study as an epidemiological marker and compared the results with those obtained with the Penner serotyping7. Thirty-four Campylobacter isolates, belonging to 10 different Penner serotypes, were included in this study. These campylobacters were isolated from broilers, other domestic animals on the farm and from darkling beetles inside the broiler houses.
KeywordsHorizontal Transmission Animal Source Coli Layer Broiler Flock Broiler House
Unable to display preview. Download preview PDF.
- 3.Guerry P., Logan S.M. and Trust T.J. (1987) J. Bacteriol, 170, 316–319.Google Scholar
- 5.Jensen M.A., Webster J.A. and Straus, N. (1993) Appl. Environm. Microbiol, 59, 945–952.Google Scholar
- 9.Van de Giesen A., Mazurier S.I., Jacobs-Reitsma W.F., Jansen W., Berkers P., Ritmeester W. and Wernars K. (1992) Appl. Environm. Microbiol, 58, 1913–1917.Google Scholar