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Comparison of Selective Media for Primary Isolation of Campylobacters

  • Carlos AbeytaJr.
  • Bradley J. Tenge
  • Jan M. Hunt
  • Paula A. Trost
  • Don H. Bark
  • Charles A. Kaysner
  • Marleen M. Wekell

Abstract

Many different types of selective media have been developed3–5, 7, 9, 11, 12. The foremost characteristic of an effective (“high performance”) plating media is the support of the growth of a wide range of C. jejuni strains with inhibition of the growth of other unwanted bacteria (background microbial flora). Background flora associated with Campylobacter is dependent upon the sample matrix2. Campylobacter-selective, high performance media were based on the ability of the media to recover the most campylobacters (“high, target signal”) with least colonization by background contaminants (“low, competitive signal. Plate counts are the scores of distinct values for colonization by Campylobacter (“target signal” C for Campylobacter percent recovery) versus colonization by flora (“competitive signal” F for flora percent recovery). The calculated test measure differential recovery% is differential recovery %=C − F(Eqn. 1). The ratiometric recovery is ratiometric recovery = C/(C+F) (Eqn. 2). The differential recovery% value approaching 100 represents overwhelming growth of Campylobacter in the presence of little or no flora. However this test measure may not resolve situations in which there is equivalent suppression of Campylobacter growth or enhancement flora colonization. The ratiometric measure used in tandem with the differential measure resolves this redundancy. Most of the trends in variation in the data were visualized using differential recovery% side-by-side range boxplots6,10.

Keywords

Selective Medium Milk Matrice Inoculation Level Ratiometric Measure Left Hand Quadrant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Abeyta. C. Jr., and Kaysner, C. A. (1991). Abstr. of the Vlth Workshop on Campylobacter, Helicobacter, and Related Organisms. Microb. Ecol. Health Dis. 4, S41.CrossRefGoogle Scholar
  2. 2.
    Abeyta, C. Jr., Kaysner, C. A., Hunt, J. M., and Wekell, M. M. (1995) J. Rapid Meth. & Automation in Microb. 4, 51–64.CrossRefGoogle Scholar
  3. 3.
    Blaser, M. J., and Barth Relier, L. (1981) N. Engl. J. Med. 305, 1444–1452.PubMedCrossRefGoogle Scholar
  4. 4.
    Bolton, F. J., and Robertson, L. (1982) J. Clin. Pathol. 35, 462–467.PubMedCrossRefGoogle Scholar
  5. 5.
    Bolton, F. J., and Coates, D. (1983) J. Appl. Bact. 54. 115–125.CrossRefGoogle Scholar
  6. 6.
    Box, G.E.P., Hunter, W.G., and Hunter, W.G. (1978). “Statistics for Experimenters.” New York: John Wiley and Sons, pp. 165–287.Google Scholar
  7. 7.
    Butzler, J. P., and Skirrow, M. B. (1979) Clin. Castro. 8, 737–765.Google Scholar
  8. 8.
    FDA (1992) “Isolation of Campylobacter species” in “Bacteriological Analytical Manual, 7th Edition.” Association of Official Analytical Chemists, ed., Arlington, VA., pp. 77-94.Google Scholar
  9. 9.
    Goossens, H., De Boeck, M., and Butzler, J. P. (1983) Fur. J. Clin. Microbiol. 2, 389–394.CrossRefGoogle Scholar
  10. 10.
    Hoaglin, D. C., Mosteller, F., Tukey, J. W., eds. “Fundamentals of Exploratory Analysis of Variance,” New York: John Wiley and Sons, 1978, pp. 24–71.Google Scholar
  11. 11.
    Karmali, M. A., Simor, A. E., Roscoe, M., Fleming, P. C., Smith, S. S. and Lane, J. (1986) J. Clin. Microbiol. 23, 456–459.PubMedGoogle Scholar
  12. 12.
    Skirrows, M. B. (1977) Br. Med. 2, 9–11.CrossRefGoogle Scholar
  13. 13.
    Stern, N. J., Wojton, B., and Kwiatek, K. (1992) J. Food Prot. 55, 514–517.Google Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Carlos AbeytaJr.
    • 1
  • Bradley J. Tenge
    • 1
  • Jan M. Hunt
    • 1
  • Paula A. Trost
    • 1
  • Don H. Bark
    • 1
  • Charles A. Kaysner
    • 1
  • Marleen M. Wekell
    • 1
  1. 1.Seafood Product Research CenterU. S. Food and Drug AdministrationBothellUSA

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