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A Multi-Centre Study of Methods for Sub-Typing Campylobacter jejuni

  • F. J. Bolton
  • A. J. Fox
  • J. Gibson
  • R. H. Madden
  • J. E. Moore
  • L. Moran
  • P. Murphy
  • R. J. Owen
  • T. H. Pennington
  • T. Stanley
  • F. Thompson-Carter
  • D. R. A. Wareing
  • T. Wilson

Abstract

A multi-centre collaborative study was carried out to compare, established sub-typing techniques for C. jejuni based upon phenotypic markers namely serotype, phagetype and biotype with molecular typing methods such as 16S rRNA RFLP’s, pulsed field gel electrophoresis, RAPD, PCR-RFLP and multilocus enzyme electrophoresis, for their relative discriminatory sensitivities and specificities.

Keywords

Restriction Fragment Length Polymorphism Outbreak Strain Enzyme Electrophoresis Restriction Fragment Length Polymorphism Typing Molecular Typing Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • F. J. Bolton
    • 1
  • A. J. Fox
    • 2
  • J. Gibson
    • 3
  • R. H. Madden
    • 4
  • J. E. Moore
    • 5
  • L. Moran
    • 4
  • P. Murphy
    • 5
  • R. J. Owen
    • 3
  • T. H. Pennington
    • 6
  • T. Stanley
    • 5
  • F. Thompson-Carter
    • 6
  • D. R. A. Wareing
    • 1
  • T. Wilson
    • 5
  1. 1.Preston Public Health LaboratoryRoyal Preston HospitalPrestonUK
  2. 2.Manchester Public Health LaboratoryWithington HospitalManchesterUK
  3. 3.National Collection of Type CulturesLondonUK
  4. 4.Department of Food MicrobiologyQueen’s University of BelfastBelfastUK
  5. 5.Department of Medical MicrobiologyBelfast City HospitalBelfastUK
  6. 6.Department of Medical MicrobiologyAberdeen Royal InfirmaryForesterhill, AberdeenUK

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