Advertisement

PCR/RFLP and PFGE Sub-Typing of Thermophilic Campylobacter Isolates from Poultry Epidemiological Investigations

  • R. D. Ayling
  • L. E. Johnson
  • S. Evans
  • D. G. Newell

Abstract

Epidemiological studies are urgently required to determine the sources and routes of transmission of campylobacter infections in broiler houses so that suitable intervention methods can be developed3. Recently a molecular typing scheme has been established using Restriction Fragment Length Polymorphism (RFLP) analysis of Polymerase Chain Reaction (PCR) products of the flagellin A and B genes1. The aim of this study was to use this technique and PFGE to analyse subtypes of C. jejuni in a significant proportion (about 10%) of the poultry flock in Great Britain.

Keywords

Cloacal Swab Typical PFGE PFGE Profile Single Point Source Poultry Flock 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ayling, R. D., Woodward, M. J., Evans, S. J., Newell, D. G., (1996) Res. Vet. Sci. 60, 168–172.PubMedCrossRefGoogle Scholar
  2. 2.
    De Boer, E., & Humphrey, T. J. (1991) Microbial Ecology in Health and Diseases. 4. (special issue): S43.Google Scholar
  3. 3.
    Evans, S. J. (1992) Vet. Record. 131, 574–576.Google Scholar
  4. 4.
    Eyers, M., Chapelle, S., Van Camp, G., Goosens, H., De Wachter, R., (1994) J. Clin Microbiol. 31, p3340–3343.Google Scholar
  5. 5.
    Gibson, J. R., Sutherland, K., Owen, R. J., (1994) Letters in Applied Microbiology, 19.Google Scholar
  6. 6.
    Koenraad, P. M. F. J., Ayling, R., Hazeleger, W. C, Rombouts, F. M., Newell, D. G. (1995). Epidem. & Infect. (In Press).Google Scholar
  7. 7.
    Skirrow, M. B. (1977) British Medical Journal 2: 9–11.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • R. D. Ayling
    • 1
  • L. E. Johnson
    • 1
  • S. Evans
    • 1
  • D. G. Newell
    • 1
  1. 1.Central Veterinary Laboratory (Weybridge)New Haw, Addlestone, SurreyUK

Personalised recommendations