Isolation of Sub-Lethally Injured Campylobacters from Water
Water is an important source of campylobacters and has been responsible worldwide for many outbreaks of enteritis.18 Campylobacters in water may be sub-lethally injured by low temperature4,19 osmotic stress13, nutrient depletion12 and/or competition from other organisms. It is also alleged that they may enter a physiological state where they are “viable but non-culturable”19 with the potential to infect animals and humans. The main manifestations of sub-lethal injury are an inability to grow at 42°C8, increased sensitivity to antimicrobials9, sensitivity to hydrogen peroxide6 and photochemically induced toxic oxygen radicals.5 These sensitivities mean that sensitive isolation techniques which take injury into account must be used.
KeywordsInjured Cell Ferrous Sulphate Public Health Laboratory Sodium Metabisulphite Unit Public Health
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