Chemical Structures of Lipopolysaccharides
Chemical characterizations of lipopolysaccharides (LPS) were undertaken to seek a molecular basis for understanding strain to strain antigenic variations and the interactions of these complex bacterial cell surface components with those of gastric mucosal cells. The surface location and wide diversity of oligosaccharide units in complex glycoconju-gates renders them well positioned to participate in cell-cell recognition events.3 LPS are markers for antigenic variations in Gram-negative bacteria, are prime candidates for the mediation of adhesion to human cell surfaces, and there is evidence for roles of H. pylori LPS in disruption of the integrity of the gastric mucosa by binding to the mucosal mucin receptor6 and in the stimulation of pepsinogen leading to penetration of the gastric epithelium by the action of pepsin.7 Evidence for antigenic diversity in H. pylori LPS4 and for strain-specific differences in the ability of the organism to bind to gastric mucosa2 prompted our study of the detailed chemical structures from antigenically different strains of H. pylori (formerly Campylobacter pylori, but now classified in a separate genus) in extension of a 5 year collaboration with Dr J. L. Penner (Dept. of Microbiology, Univ. Toronto) on LPS from Campylobacter species. Advantage was also taken of another ongoing collaboration with Dr. A. P. Moran (Department of Microbiology, University College, Galway) on Campylobacter LPS, to extend his previous investigations H. pylori LPS from rough-form5 to smooth-form LPS.
KeywordsSugar Hydrolysis Carbohydrate Oligosaccharide GlcNAc
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