The Usefulness of the Chick Colonisation Model to Investigate Potential Colonisation Factors of Campylobacters
The mechanisms of the pathogenesis of Campylobacter jejuni/coli remain largely unknown. Current approaches to the identification of virulence and colonisation factors frequently involve producing a gene knockout mutant, which then requires testing in in vivo or in vitro models. Unfortunately, there is to date no model of human campylobacteriosis. However, the avian gastro-intestinal tract is considered the natural environment of the thermophilic campylobacters. The true importance of these genes can then be seen by testing the deletion mutants in vivo. The chick, therefore, represents an appropriate model for testing genetically-engineered mutants for their ability to colonise and survive in vivo.
KeywordsParent Strain Maximum Colonisation Colonisation Potential Isogenic Mutant Caecal Content
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