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Characterization of Flagellin from Campylobacter Jejuni with Monoclonal Antibody C3G8

Possible Recognition of Post-Translational Modification Epitope
  • R. Meinersmann
  • K. Hiett
  • M. Myszewski
  • M. Carr
  • S. Cawthraw
  • N. Stern
  • D. Newell

Abstract

Flagellin has been identified as an immunodominant constituent of Campylobacter jejuni 4. The flagella have been known to undergo antigenic variation2 and multiple charge trains of flagellin protein are seen in two-dimensional electrophoresis5. Protein sequencing analysis of flagellin has suggested that it may be phosphorylated2 and recently Doig et al.1 have presented evidence for glycosylation of flagellin.

Keywords

Outer Membrane Protein Flagellin Gene Sodium Metaperiodate Standard Western Blot Flagellin Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Doig P., Kinsella N. M., Guerry P., and Trust T. J. (1995) Abstracts 95th ASM Gen Mtg pp. 180.Google Scholar
  2. 2.
    Harris J.R., Logan S. M., Guerry P., and Trust T. J. (1987) J Bacteriol 169:5066.PubMedGoogle Scholar
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    Logan R.P., Trust T.J., and Guerry P. (1989) J Bacteriol 171:3031.PubMedGoogle Scholar
  4. 4.
    Nachamkin I., and Hart A.M. (1985) J Clin Microbiol 21:33.PubMedGoogle Scholar
  5. 5.
    Nachamkin I., and Yang X.H. (1988) FEMS Microbiol Lett 49:235.CrossRefGoogle Scholar
  6. 6.
    Nuijten P. J., van der Zeijst B. A., and Newell D. G. (1991) Infect Immun 59:1100.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • R. Meinersmann
    • 1
  • K. Hiett
    • 1
  • M. Myszewski
    • 1
  • M. Carr
    • 1
  • S. Cawthraw
    • 2
  • N. Stern
    • 1
  • D. Newell
    • 2
  1. 1.Agricultural Research ServiceUSDAAthensUSA
  2. 2.Central Veterinary LaboratoryNew Haw Addlestone, SurreyUK

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