Natural Transformation as a Tool for the Characterization of Motility Mutants of Campylobacter Jejuni 81116

  • Ben N. Fry
  • Trudy M. Wassenaar
  • Robbert Zalm
  • Bernard A. M. van der Zeijst


The flagella of Carnpylobacter jejuni play an important role in pathogenicity. Wassenaar et al. 18 and Grant et al. 3 showed that flagella are important for invasion, while Ruiz-Palacios et al. 14 showed that invasiveness is associated with inflammatory diarrhoea. Motility, rather than the presence of flagella, is the major factor involved in invasion18,3 Furthermore, flagella play an important role in colonisation19,4,12,1,6. The presence of flagellin A, rather than motility, seems to be responsible for optimal bacterial colonization of chicken caeca19.


Natural Transformation Microaerophilic Condition Flagellin Gene Motility Mutant flaA Gene 
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  1. 1.
    Aguero-Rosenfeld M.E., Yang X.H. and Nachamkin I. (1990) Infect. Immun. 58, 2214–9.PubMedGoogle Scholar
  2. 2.
    Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A. and Struhl K. (eds.) (1994) Current Protocols in Molecular Biology. Jon Wiley & Sons, Inc.Google Scholar
  3. 3.
    Grant C.C., Konkel M.E., Cieplak W.J. and Tompkins L.S. (1993) Infect. Immun. 61, 1764–71.PubMedGoogle Scholar
  4. 4.
    Nachamkin I., Yang X.H. and Stern N.J. (1993) Appl. Environ. Microbiol. 59, 1269–73.PubMedGoogle Scholar
  5. 5.
    Newell D.G., McBride H. and Pearson A.D. (1984) J. Gen. Microbiol. 130, 1201–1208.PubMedGoogle Scholar
  6. 6.
    Newell D.G., McBride G. and Dolby J.M. (1985) J. Hyg. Camb. 95, 217–227.PubMedCrossRefGoogle Scholar
  7. 7.
    Newell D.G. (1986) J. Hyg. Camb. 96, 377–384.PubMedCrossRefGoogle Scholar
  8. 8.
    Nuijten P.J.M., Bleumink-Pluym N.M.C., Gaastra W. and Van der Zeijst B.A.M. (1989) Infect. Immun. 57, 1084–1088.PubMedGoogle Scholar
  9. 9.
    Nuijten P.J.M., Van Asten A.J.A.M., Gaastra W. and Van der Zeijst B.A.M. (1990) J. Biol. Chem. 265, 17798–17804.PubMedGoogle Scholar
  10. 10.
    Nuijten P.J.M., Van der Zeijst B.A.M. and D. G. Newell (1991) Infect. Immun. 59, 1100–5.PubMedGoogle Scholar
  11. 11.
    Palmer S.R., Gully P.R., White J.M., Pearson A.D., Suckling W.G, Jones D.M., Rawes J.C.L. and Penner J.L. (1983) Lancet i, 287–290.CrossRefGoogle Scholar
  12. 12.
    Pavlovskis O.R., Rollins D.M., Haberberger R.J., Green A.E., Habash L., Strocko S. and Walker R.I. (1991) Infect. Immun. 59, 2259–64.PubMedGoogle Scholar
  13. 13.
    Rottier P.J.M., Spaan W.J.M., Horzinek M.C. and Van der Zeijst B.A.M. (1981) J. Virol. 38, 20–26.PubMedGoogle Scholar
  14. 14.
    Ruiz-Palacios G.M., Cervantes L.E., Newburg D.S., Lopez-Vidal Y. and Calva J.J. (1992) In: Campylobacter jejuni Current Status and Future Trends. Nachamkin I., Blaser M.J. and Tompkins L.S. (eds.) ASM., Washington DC. pp. 176–183.Google Scholar
  15. 15.
    Sambrook J., Fritsch E.F. and Maniatis T. (1989) Molecular Cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.Google Scholar
  16. 16.
    Taylor D.E., Hiratsuka K., Ray H., Manavathu E.K. (1987) J. Bacteriol. 169, 2984–2989.PubMedGoogle Scholar
  17. 17.
    Wang Y. and Taylor D. (1990) J. Bacteriol. 172, 949–955.PubMedGoogle Scholar
  18. 18.
    Wassenaar G.M., Bleumink-Pluym N.M.C. and Van der Zeijst B.A.M. (1991) EMBO J. 8, 2055–2061.Google Scholar
  19. 19.
    Wassenaar G.M., Van der Zeijst B.A.M., Ayling R. and Newell D.G. (1993) J. Gen. Microbiol. 139, 1171–1175.PubMedCrossRefGoogle Scholar
  20. 20.
    Wassenaar G.M., Fry B.N. and Van der Zeijst B.A.M. (1993) Gene 132, 131–135.PubMedCrossRefGoogle Scholar
  21. 21.
    Wassenaar G.M., Fry B.N. and Van der Zeijst B.A.M. (1995) Microbiol. 141, 95–101.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Ben N. Fry
    • 1
  • Trudy M. Wassenaar
    • 1
  • Robbert Zalm
    • 1
  • Bernard A. M. van der Zeijst
    • 1
  1. 1.Department of Bacteriology, Institute of Infectious Diseases and Immunology, School of VeterinaryMedicine UniversiteitUtrechtThe Netherlands

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