Local Protein Synthesis in the Squid Giant Axon and Presynaptic Terminals
The existence of a local system of axonal protein synthesis was proposed approximately 35 years ago to explain the similar rates of reappearance of acetylcholinesterase activity in proximal and distal regions of the cat hypoglossal nerve following irreversible inactivation of the enzyme (Koenig and Koelle, 1960). Since then this question has been approached with more direct techniques, by measuring the incorporation of radiolabelled aminoacids into the proteins of the axonal compartment following its separation from the closely apposed glial cells by microdissection or by autoradiographic methods (Koenig, 1984; Giuditta et al., 1990). The separation of axonal and glial compartments is more readily accomplished in large axons, such as the squid giant axon (Giuditta et al., 1968; 1983; 1991) and the Mauthner axon of the goldfish (Koenig, 1979; 1991; Koenig and Martin, 1996).
KeywordsCellulose Sucrose Phosphorus Sedimentation Methionine
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