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Serological Subtypes of Botulinal Neurotoxins

  • Chapter
Botulinum and Tetanus Neurotoxins

Abstract

The taxonomy of botulinal bacteria, as responsible for synthesizing botulinal neurotoxin (BoNT), embraces three different but interrelated subjects: (1) the systematic of Clostridium botulinum and its physiologic and genetic related bacteria;1 (2) other clostridial species producing BoNT,2,3,4,5 and (3) the BoNT. This toxin has a unique pharmacological action, the blockade of the neurotransmitter acetylcholine release from the nerve terminal.6

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References

  1. L.V. Holdeman and J.B. Brooks, Variation among strains of Clostridium botulinum and related clostridia, in: “Proceedings of the First U.S. Japan Conference on Toxic Microorganism,” M. Herzberg, ed., U.S. Govt. Printing Office, Washington, D. C. (1970).

    Google Scholar 

  2. M.W. Eklund, F.T. Poysky, J.A. Meyers and G.A. Pelroy, Interspecies conversion of Clostridium botulinum type C to Clostridium novyi type A by bacteriophage, Science. 186: 456 (1974).

    Article  PubMed  CAS  Google Scholar 

  3. J.D. Hall, L.M. McCroskey, B.J. Pincomb and C.L. Hatheway, Isolation of an organism resembling Clostridium barati which produces type F botulinal toxin from an infant with botulism, J. Clin. Microbial. 21: 654 (1985).

    CAS  Google Scholar 

  4. L.M. McCroskey, C.L. Hatheway, L. Fenicia, B. Pasolini and P. Aureli, Characterization of an organism that produces type E botulinal toxin but which resembles Clostridium butyricum from the feces of an infant with type E botulism, J. Clin. Microbiol. 23: 201 (1986).

    PubMed  CAS  Google Scholar 

  5. J.C. Suen, C.L. Hatheway, A.G. Steigerwalt and D.J. Brenner, Clostridium argentinense sp. nov.: a genetically homogeneous group composed of all strains of Clostridium botulinum toxin type G and some nontoxigenic strains previously identified as Clostridium subterminale or Clostridium hastiforme, Intern. J. Syst. Bacteriol. 38: 375 (1988).

    Article  Google Scholar 

  6. L.C. Sellin, Botulinum toxin and the blockade of transmitter release, Asia Pacific J. Pharmacol. 2: 203 (1987).

    CAS  Google Scholar 

  7. D.F. Giménez and A.S. Ciccarelli, Variaciones antigénicas en toxins del tipo F. Ensayo de definiciones para la tipificación serológica y clasificación de Clostridium botulinum, Medicina (Bs Aires). 32: 596 (1972).

    Google Scholar 

  8. D.F. Giménez and A.S. Ciccarelli, A new type of Cl. botulinum, in: “Botulism 1966, ” M. Ingram and T.A. Roberts, ed., Chapman and Hall, London (1967).

    Google Scholar 

  9. D.F. Giménez and A.S. Ciccarelli, Studies on strain 84 of Clostridium botulinum, Zbl. Bakt., I. Abt. Orig. 215: 212 (1970).

    Google Scholar 

  10. C.L. Hatheway, L.M. McCroskey, G.L. Lombard and V.R. Dowell Jr., Atypical toxin variant of Clostridium botulinum type B associated with infant botulism, J. Clin. Microbiol. 14: 607 (1981).

    PubMed  CAS  Google Scholar 

  11. D.F. Giménez and J.A. Giménez, Identificación de la cepa B 657 de Clostridium botulinum, Rev. Argent. Microbiol. 15: 51 (1983).

    PubMed  Google Scholar 

  12. D.F. Giménez, Clostridium botulinum subtype Ba, Zbl. Bakt. Hyg. A 257: 68 (1984).

    Google Scholar 

  13. M. Poumeyrol, J. Billon, F. Delille, C. Haas, A. Marmonier and M. Sebald, Intoxication botulique mortelle due a une souche de Clostridium botulinum de type AB, Med. Mal. Inf. 13: 750 (1983).

    Article  Google Scholar 

  14. G. Sakaguchi, S. Sakaguchi, S. Kozaki and M. Takahashi, Purification and some properties of Clostridium botulinum type AB toxin, FEMS Microbiol. Letters 33: 23 (1986).

    Article  CAS  Google Scholar 

  15. L.M. McCroskey and C.L. Hatheway, Atypical strains of Clostridium botulinum isolated from specimens in infant botulism cases, ASM Meet. (abs.). C 159: 263 (1984).

    Google Scholar 

  16. L.DS. Smith and L.V. Holdeman, “The Pathogenic Anaerobic Bacteria,” C. Thomas, Springfield, Illinois (1968).

    Google Scholar 

  17. L.DS. Smith and G. Hobbs, Clostridium, in: “Bergey’s Manual of Determinative Bacteriology,” R.B. Buchanan and N.E. Gibbons, ed., Williams and Wilkins, Baltimore (1974).

    Google Scholar 

  18. W.H. Lee and H. Riemann, Correlation of toxic and non-toxic strains of Clostridium botulinum by DNA composition and homology, J. Gen. Microbiol. 60: 117 (1970).

    Article  PubMed  CAS  Google Scholar 

  19. W.H. Lee and H. Riemann, The genetic relatedness of proteolytic Clostridium botulinum strains, J. Gen. Microbiol. 64: 85 (1970).

    Article  PubMed  CAS  Google Scholar 

  20. C.S. Cummins, Cell wall composition in the classification of Gram positive anaerobes, Intern. J. Syst. Bacteriol. 20: 413 (1970).

    Article  Google Scholar 

  21. J.L. Johnson and B.S. Francis, Taxonomy of the clostridia: ribosomal ribonucleic acid homologies among the species, J. Gen. Microbiol. 88: 229 (1975).

    Article  PubMed  CAS  Google Scholar 

  22. M.W. Eklund and F.T. Poysky, Interconversion of type C and D strains of Clostridium botulinum by specific bacteriophage, Appl. Microbiol. 27: 251 (1974).

    CAS  Google Scholar 

  23. C.S. Gutteridge, B.M. Mackey and J.R. Norris, A pyrolysis gas-liquid chromatography study of Clostridium botulinum and related organisms, J. Appl. Bacteriol. 49: 165 (1980).

    Article  PubMed  CAS  Google Scholar 

  24. H.M. Solomon, R.K. Lynt, D.A. Kautter and T. Lilly, Antigenic relationship among the proteolytic and nonproteolytic strains of Clostridium botulinum, App!. Microbio!. 21: 295 (1971).

    CAS  Google Scholar 

  25. J.C. Suen, C.L. Hatheway, A.G. Steigerwalt and DJ. Brenner, Genetic confirmation of identities of neurotoxigenic Clostridium baratii and Clostridium butyricum implicated as agents of infant botulism, J. Clin. Microbiol. 26: 2191 (1988).

    PubMed  CAS  Google Scholar 

  26. J.A. Giménez, M.A. Giménez and B.R. DasGupta, Characterization of the neurotoxin isolated from a Clostridium baratii strain implicated in infant botulism, Infect. Immun. 60: 518 (1992).

    PubMed  Google Scholar 

  27. J.A. Giménez and H. Sugiyama, Comparison of toxins of Clostridium butyricum and Clostridium botulinum type E, Infect. Immun. 56: 926 (1988).

    PubMed  Google Scholar 

  28. J.A. Giménez, J. Foley and B.R. DasGupta, Neurotoxin type E from Clostridium botulinum and C. butyricum: partial sequence and comparison, FASEB J. 2: A1750 (1988).

    Google Scholar 

  29. B.R. Singh, J.A. Giménez and B.R. DasGupta, Comparative Molecular Topography of Botulinum Neurotoxins from Clostridium butyricum and Clostridium botulinum type E, Biochem.Biophys. Acta. 1077: 119 (1991).

    CAS  Google Scholar 

  30. S. Kozaki, J. Onimaru, Y. Kamata and G. Sakaguchi, Immunological characterization of Clostridium butyricum neurotoxin and its trypsin-induced fragment by use of monoclonal antibodies against Clostridium botulinum type E neurotoxin, Infect. Immun. 59: 457 (1991).

    PubMed  CAS  Google Scholar 

  31. EJ. Bowmer, Preparation and assay of the international standards for Clostridium botulinum types A, B, C, D and E antitoxins, Bull. Wld Hlth Org. 29: 701 (1963).

    CAS  Google Scholar 

  32. AJ. Fulthorpe, Clostridium botulinum types A and B toxin-antitoxin reactions, J. Hyg. (Gond.). 53: 180 (1955).

    Article  CAS  Google Scholar 

  33. H. Sugiyama, Clostridium botuliunum neurotoxin, Microbiol. Rev. 44: 419 (1980).

    Google Scholar 

  34. G. Sakaguchi, Clostridium botulinum toxins, Pharmac. Ther. 19: 165 (1983).

    Google Scholar 

  35. P. Hambleton, C.C. Shone and J. Melling, Botulinum toxin. Structure, action and clinical uses, in: “Neurotoxin and Their Pharmacological Implications,” P. Jenner, ed., Raven Press, New York (1987).

    Google Scholar 

  36. P. Hambleton, C.C. Shone, P. Wilton-Smith and J. Melling, A possible common antigen on clostridial toxins detected by monoclonal anti-botulinum neurotoxin antybodies, in: “Bacterial Protein Toxins,” J.E. Alouf, F.J. Fehrenbach, J.H. Freer and J. Jeljaszewic, ed., Academic Press, Inc., London (1984).

    Google Scholar 

  37. K. Tsuzuki, N. Yokosawa, B. Syuto, I. Ohishi, N. Fujii, K. Kimura and K. Oguma, Establishment of a monoclonal antibody recognizing an antigenic site common to Clostridium botulinum type B, Cl, D, and E toxins and tetanus toxin, Infect. Immun. 56: 898 (1988).

    PubMed  CAS  Google Scholar 

  38. C.E. Dolman and L. Murakami, Clostridium botulinum type F with recent observations on other types, J. Infect. Dis. 109: 107 (1961).

    Article  CAS  Google Scholar 

  39. T.I. Bulatova and E.V. Perova, Antigenic structure of Cl. botulinum, types E and F, Zh. Mikrobiol. Epidemiol. Immuno. 4: 28 (1970).

    Google Scholar 

  40. J.H. Mason and E.M. Robinson, The antigenic components of the toxins of Cl. botulinum types C and D, Onderstepoort J. Vet. Sci. Anim. Ind. 5: 65 (1935).

    CAS  Google Scholar 

  41. D.F. Giménez and A.S. Ciccarelli, Another type of Clostridium botulinum, Zbl. Bakt., I. Abt. Orig. 215: 221 (1970).

    Google Scholar 

  42. A.S. Ciccarelli and D.F. Giménez, Estudio serológico de toxinas de Clostridium botulinum tipo A y cepa 84, Rev. lat-amer. Microbiol. 13: 67 (1971).

    CAS  Google Scholar 

  43. T. Shimizu and H. Kondo, Immunological difference between the toxin of a proteolytic strain and that of a non-proteolytic strain of Clostridium botulinum type B, Jpn. J. Med. Sci. Biol. 26: 269 (1973).

    PubMed  CAS  Google Scholar 

  44. D. Rymkiewicz, J. Sawicki and A. Brühl, Study on the immunological heterogeneity of Clostridium botulinum B type toxin, Arch. Immunol. Ther. Exp. 27: 709 (1979).

    CAS  Google Scholar 

  45. C. Glasby and C.L. Hatheway, Fluorescent-antibody reagents for the identification of Clostridium botulinum, J. Clin. Microbiol. 18: 1378 (1983).

    PubMed  CAS  Google Scholar 

  46. T.I. Bulatova and K.I. Matveev, An antigenic structure of Cl. botulinum, type C strains, isolated in the USSR, Zh. Mikrobiol. Epidemiol. Immuno. 8: 79 (1965).

    Google Scholar 

  47. M. Eklund, F. Poysky, K. Oguma, H. Iida and K. Inoue, Relationship of bacteriophages to toxin and hemagglutinin production by Clostridium botulinum types C and D and its significance in avian botulism outbreaks, in: “Avian Botulism: An International Perspective,” M.W. Eklund and V.R. Dowell Jr., ed., C. Thomas, Springfield, Illinois (1987).

    Google Scholar 

  48. B.C. Jansen, Clostridium botulinum type C, its isolation, identification and taxonomic position, in: “Avian Botulism: An International Perspective,” M.W. Eklund and V.R. Dowell Jr., ed., C. Thomas, Springfield, Illinois (1987).

    Google Scholar 

  49. I. Ohishi and B.R. DasGupta, Molecular structure and biological activities of Clostridium botulinum C2 toxin, in: “Avian Botulism: An International Perspective,” M.W. Eklund and V.R. Dowell Jr., ed., C. Thomas, Springfield, Illinois (1987).

    Google Scholar 

  50. W. Pfenninger, Toxico-immunologic and serologic relationship of B. botulinus, type C, and B. parabotulinus, “Seddon”. XXII, J. Infect. Dis. 35: 347 (1924).

    Article  CAS  Google Scholar 

  51. J.B. Gunnison and K.F. Meyer, Cultural study of an international collection of Clostridium botulinum and parabotulinum. XXXVIII, J. Infect. Dis. 45: 119 (1929).

    Article  Google Scholar 

  52. B.C. Jansen, The toxic antigenic factors produced by Clostridium botulinum types C and D, Onderstepoort J. vet. Res. 38: 93 (1971).

    PubMed  CAS  Google Scholar 

  53. I. Ohishi, M. Iwasaki and G. Sakaguchi, Vascular permeability activity of botulinum C2 toxin elicited by cooperation of two dissimilar protein components, Infect. Immun. 31: 890 (1980).

    Google Scholar 

  54. B.C. Jansen and P.C. Knoetze, The taxonomic position of Clostridium botulinum type C, Onderstepoort J. vet. Res. 44: 53 (1977).

    CAS  Google Scholar 

  55. K. Oguma, B. Syuto, T. Agui, H. Iida and S. Kubo, Homogeneity and heterogeneity of toxins produced by Clostridium botulinum type C and D strains, Infect. Immun. 34: 382 (1981).

    PubMed  CAS  Google Scholar 

  56. K. Oguma, B. Syuto, H. Iida and S. Kubo, Antigenic similarity of toxins produced by Clostridium botulinum type C and D strains, Infect. Immun. 30: 656 (1980).

    CAS  Google Scholar 

  57. O.J. Ochanda, B. Syuto, K. Oguma, H. Iida and S. Kubo, Comparison of antigenicity of toxins produced by Clostridium botulinum type C and D strains, Appl. Environ. Microbiol. 47: 1319 (1984).

    CAS  Google Scholar 

  58. K. Oguma, S. Murayama, B. Syuto, H. Iida and S. Kubo, Analysis of antigenicity of Clostridium botulinum type Cl and D toxins by polyclonal and monoclonal antibodies, Infect. Immun. 43: 584 (1984).

    CAS  Google Scholar 

  59. J. Terajima, B. Syuto, J.0. Ochanda and S. Kubo, Purification and characterization of neurotoxin produced by Clostridium botulinum type C 6813, Infect. Immun. 48: 312 (1985).

    CAS  Google Scholar 

  60. K. Moriishi, B. Syuto, S. Kubo and K. Oguma, Molecular diversity of neurotoxins from Clostridium botulinum type D strains, Infect. Immun. 57: 2886 (1989).

    CAS  Google Scholar 

  61. W.B. Gross and L.DS. Smith, Experimental botulism in gallinaceous birds, Avian Dis. 15: 716 (1971).

    Article  PubMed  CAS  Google Scholar 

  62. J. Haagsma, Laboratory investigation of botulism in wild birds, in: “Avian Botulism: An International Perspective,” M.W. Eklund and V.R. Dowell Jr., ed., C. Thomas, Springfield, Illinois (1987).

    Google Scholar 

  63. A.R. Prévot, A. Turpin and P. Kaiser, “Les Bactéries Anaérobies,” Dunod, Paris (1967).

    Google Scholar 

  64. A.H.W. Hauschild, Clostridium botulinum toxins, Intern. J. Food Microbiol. 10: 113 (1990).

    Article  CAS  Google Scholar 

  65. H. Sugiyama, K. Mizutani and K.H. Yang, Basis of type A and F toxicitites of Clostridium botulinum strain 84, Proc. Soc. Exp. Biol. and Med. 141: 1063 (1972).

    Article  CAS  Google Scholar 

  66. B.R. DasGupta and A. Datta, Botulinum neurotoxin type B (strain 657): partial sequence and similarity with tetanus toxin, Biochimie 70: 811 (1988).

    Article  PubMed  CAS  Google Scholar 

  67. B.J. Edmond, F.A. Guerra, J. Blake and S. Hempler, Case of infant botulism in Texas, Tex. Med. 73: 85 (1977).

    PubMed  CAS  Google Scholar 

  68. C.L. Hatheway and L.M. McCroskey, Examination of feces and serum for diagnosis of infant botulism in 366 patients, J. Clin. Microbiol. 25: 2334 (1987).

    PubMed  CAS  Google Scholar 

  69. C.L. Hatheway and L.M. McCroskey, Unusual neurotoxigenic clostridia recovered from human fecal specimens in the investigation of botulism, in: “Recent Advances in Microbial Ecology,” T. Hattori, Y. Ishida, Y. Maruyama, R.Y. Morita and A. Uchida, ed., Proceedings of the 5th International Symposium on Microbial Ecology. Japan Scientific Societies Press, Tokyo (1989).

    Google Scholar 

  70. R.A. Fernández, A.S. Ciccarelli, G.N. Arenas and D.F. Giménez, Primer brote de botulismo por Clostridium botulinum subtipo Af, Rev. Argent. Microbiol. 18: 29 (1986).

    PubMed  Google Scholar 

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Giménez, D.F., Giménez, J.A. (1993). Serological Subtypes of Botulinal Neurotoxins. In: DasGupta, B.R. (eds) Botulinum and Tetanus Neurotoxins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9542-4_46

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  • DOI: https://doi.org/10.1007/978-1-4757-9542-4_46

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