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Strain Improvement and Process Development

  • Giancarlo Lancini
  • Rolando Lorenzetti

Abstract

The productivity of strains isolated in a screening program is ordinarily very low; commonly a few milligrams of metabolite per liter of culture. Initial evaluation of the biological potential of a new metabolite and its chemical characterization requires grams of pure material. Kilograms and tens of kilograms are then needed for animal toxicology studies and later for clinical trials. Therefore, from the initial stages of the development of a new metabolite it is necessary to improve the productivity of the fermentation process. In later stages of development the increase of yields is of paramount importance to define an industrial process by which the substance can be produced at an acceptable cost.

Keywords

Protoplast Fusion Antibiotic Production Strain Improvement Mutagenic Treatment Flask Fermentation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baltz, R. H., 1986, Mutagenesis in Streptomyces spp., in Manual of Industrial Microbiology and Biotechnology ( A. L. Demain and N. A. Solomon, eds.), pp. 184–190, American Society for Microbiology, Washington, D.C.Google Scholar
  2. Calam, C. T., 1986, Physiology of the overproduction of secondary metabolites, in Overproduction of Microbial Metabolites. Strain Improvement and Process Control Strategies ( Z. Vanék and Z. Hostâlek, eds.), pp. 27–50, Butterworths, London.Google Scholar
  3. Chater, K. F., 1990, The improving prospects for yield increase by genetic engineering in antibiotic-producing streptomycetes, Bio/Technology 8: 115.PubMedCrossRefGoogle Scholar
  4. Elender, R. P., 1987, Microbial screening, selection and strain improvement, in Basic Biotechnology ( J. Bu’Lock and B. Kristiansen, eds.), pp. 217–251, Academic Press, New York.Google Scholar
  5. Elander, R. P., and Vournakis, J. N., 1986, Genetic aspects of overproduction of antibiotics and other secondary metabolites, in Overproduction of Microbial Metabolites. Strain Improvement and Process Control Strategies ( Z. Vanék and Z. Hostâlek, eds.), pp. 63–80, Butterworths, London.Google Scholar
  6. Holt, G., Saunders, G., and Dales, R., 1986, Genetic approaches to overproduction of fungal antibiotics, in Overproduction of Microbial Metabolites. Strain Improvement and Process Control Strategies ( Z. Vanék and Z. Hostâlek, eds.), pp. 81–104, Butterworths, London.Google Scholar
  7. Katz, L., and Hutchinson, C. R., 1992, Genetic engineering of antibiotic producing organisms, Annu. Rep. Med. Chem. 27: 129.CrossRefGoogle Scholar
  8. Matsushima, P., and Baltz, R. H., 1986, Protoplast fusion, in Manual of Industrial Microbiology and Biotechnology (A. L. Demain and N. A. Solomon, eds.), pp. 170183, American Society for Microbiology, Washington, D.C.Google Scholar
  9. Nisbet, L. J.,and Winstanley, D. J.,(eds.), 1983, Bioactive Products 2. Development and Production,Academic Press, New York.Google Scholar
  10. Normansell, I. D., 1986, Isolation of Streptomyces mutants improved for antibiotic production, in The Bacteria, Vol. IX ( S. W. Queener and L. E. Day, eds.), pp. 95118, Academic Press, New York.Google Scholar
  11. Queener, S. W., and Lively, D. H., 1986, Screening and selection for strain improvement, in Manual of Industrial Microbiology and Biotechnology ( A. L. Demain and N. A. Solomon, eds.), pp. 155–169, American Society for Microbiology, Washington, D.C.Google Scholar
  12. Rowlands, R. T., 1992, Strain improvement and strain stability, in Biotechnology of Filamentous Fungi ( B. S. Finkelstein and C. Ball, eds.), pp. 41–64, Butterworths, London.Google Scholar
  13. Saunders, V. A., and Saunders, J. R., 1987, Microbial Genetics Applied to Biotechnology. Principles and Techniques of Gene Transfer and Manipulation, Croom Helm, London.Google Scholar
  14. Skatrud, P. L., Tietz, A. J., Ingolia, T. D., Cantwell, C. A., Fisher, D. L., Chapmann, J. L., and Queener, S. W., 1989, Use of recombinant DNA to improve production of cephalosporin C by Cephalosporium acremonium, Bio/Technology 7: 477.CrossRefGoogle Scholar
  15. Stuttzman-Engwall, K. J., Otten, S. L., and Hutchinson, C. R., 1992, Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius, J. Bacteriol. 174: 144.Google Scholar
  16. Trilli, A., 1986, Scale-up of fermentations, in Manual of Industrial Microbiology and Biotechnology ( A. L. Demain and N. A. Solomon, eds.), pp. 277–307, American Society for Microbiology, Washington, D.C.Google Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Giancarlo Lancini
    • 1
  • Rolando Lorenzetti
    • 1
  1. 1.MMDRI-Lepetit Research CenterGerenzano (Varese)Italy

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