Results of thermal analyses of select diagnostic enzymes and their utility in determining stability of dry chemistries have been presented in this paper. An enzymatic compound consisting of glucose oxidase, peroxidase, tetramethyl benzidine indicator, alkylbenzene sulfonate and polyhydroxyethyl methacrylate was prepared and compression molded. Strips molded up to 150°C gave good glucose response. During molding the redox centers of the enzyme are not disturbed whereas the bulk of the enzyme, polymeric in composition, blends with PHEMA producing the unusual stability. There is a distinct possibility that a reaction injection molding (RIM) composition and processing can be developed for making dry chemistries.
KeywordsGlucose Oxidase Hydroxyethyl Methacrylate Linear Alkylbenzene Sulfonate Amino Sugar Cholesterol Oxidase
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