Time-Resolved Fluorescence in Biology and Biochemistry



A number of articles and books have appeared in recent years describing in varying detail the theory and application of time-resolved fluorescence spectroscopy in the chemical and biological sciences (Badea and Brand, 1979; Demas, 1983; Lakowicz, 1983; Cundall and Dale, 1983; Gratton et al., 1984a; Jameson et al., 1984; Taylor et al., 1987; Jameson and Reinhart, 1989). Our goal in this chapter is thus not to treat the subject exhaustively but rather to reiterate the fundamental considerations and to provide the reader with a feeling for the practical aspects that must be apprehended before time-resolved methods can be profitably applied to complex biological systems.


Fluorescence Lifetime Rotational Correlation Time Differential Phase Free Probe Modulation Ratio 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  1. 1.Department of Biochemistry and Biophysics, John A. Burns School of MedicineUniversity of Hawaii at ManoaHonoluluUSA

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