Abstract
DNA sequencing is a relatively new technique, just over a decade old. Scientifically, it is a very important technique because the DNA sequence gives us the ultimate resolution of the genetic material to the single base level and thus its complete information.
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Abbreviations
- DMSO:
-
dimethyl sulfoxide
- dATP:
-
deoxyadenosine triphosphate
- ddATP:
-
dideoxyadenosine triphosphate
- dCTP:
-
deoxycytidine triphosphate
- ddCTP:
-
dideoxycytidine triphosphate
- dGTP:
-
deoxyguanosine triphosphate
- ddGTP:
-
dideoxyguanosine triphosphate
- dNTP:
-
deoxynucleotide triphosphate
- ddNTP:
-
dideoxynucleotide triphosphate
- dTTP:
-
deoxythymidine triphosphate
- ddTTP:
-
dideoxythymidine triphosphate
- EDTA:
-
ethylenediaminetetraacetic acid
- EMBL:
-
European Molecular Biology Laboratory
- FITC:
-
fluorescein isothiocyanate
- GCG:
-
(University of Wisconsin) Genetics Computer Group
- HPLC:
-
high-pressure liquid chromatography
- O.D.:
-
optical density
- PAGE:
-
polyacrylamide gel electrophoresis
- PEG:
-
polyethylene glycol
- PMT:
-
photomultiplier tube
- RPHPLC:
-
reverse-phase high-pressure liquid chromatography
- TEMED:
-
tetramethylethylenediamine
- TES:
-
Tris-EDTA salt solution
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Brumbaugh, J., Middendorf, L., Grone, D., Ruth, J. (1991). Continuous, On-Line, Real Time DNA Sequencing Using Multifluorescently Tagged Primers. In: Dewey, T.G. (eds) Biophysical and Biochemical Aspects of Fluorescence Spectroscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9513-4_3
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