Advertisement

Continuous, On-Line, Real Time DNA Sequencing Using Multifluorescently Tagged Primers

  • John Brumbaugh
  • Lyle Middendorf
  • Dan Grone
  • Jerry Ruth

Abstract

DNA sequencing is a relatively new technique, just over a decade old. Scientifically, it is a very important technique because the DNA sequence gives us the ultimate resolution of the genetic material to the single base level and thus its complete information.

Keywords

Helper Virus Longe Fragment Genetic Computer Group dNTP Pool Binding Silane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

DMSO

dimethyl sulfoxide

dATP

deoxyadenosine triphosphate

ddATP

dideoxyadenosine triphosphate

dCTP

deoxycytidine triphosphate

ddCTP

dideoxycytidine triphosphate

dGTP

deoxyguanosine triphosphate

ddGTP

dideoxyguanosine triphosphate

dNTP

deoxynucleotide triphosphate

ddNTP

dideoxynucleotide triphosphate

dTTP

deoxythymidine triphosphate

ddTTP

dideoxythymidine triphosphate

EDTA

ethylenediaminetetraacetic acid

EMBL

European Molecular Biology Laboratory

FITC

fluorescein isothiocyanate

GCG

(University of Wisconsin) Genetics Computer Group

HPLC

high-pressure liquid chromatography

O.D.

optical density

PAGE

polyacrylamide gel electrophoresis

PEG

polyethylene glycol

PMT

photomultiplier tube

RPHPLC

reverse-phase high-pressure liquid chromatography

TEMED

tetramethylethylenediamine

TES

Tris-EDTA salt solution

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ansorge, W., and Barker, R., 1984, J. Biochem. Biophys. Methods 9:33–47.PubMedCrossRefGoogle Scholar
  2. Ansorge, W., and Labeit, S., 1984, J. Biochem. Biophys. Methods 10:237–243.PubMedCrossRefGoogle Scholar
  3. Ansorge, W., Sproat, B. S., Stegemann, J., and Schwager, C., 1986, J. Biochem. Biophys. Methods 13:315–323.PubMedCrossRefGoogle Scholar
  4. Ansorge, W., Sproat, B., Stegemann, J., Schwager, C., and Zenke, M., 1987, Nucleic Acids Res. 15(11):4593–4602.PubMedCrossRefGoogle Scholar
  5. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., 1987, Current Protocols in Molecular Biology, Greene Publishing Associates and Wiley-Interscience, New York.Google Scholar
  6. Barr, P. J., Thayer, R. M., Laybourn, P., Najarian, R. C., Seela, F., and Tolan, D. R., 1986, BioTechniques 4(5):428–431.Google Scholar
  7. Beck, S., 1987, , Anal. Biochem. 164(2):514–520.PubMedCrossRefGoogle Scholar
  8. Beck, S., and Pohl, F. M., 1984, EMBO J. 3:2905–2909.PubMedGoogle Scholar
  9. Biggen, M. D., Gibson, T. J., and Hong, G. F., 1983, Proc. Natl. Acad. Sci. USA 80:3963–3965.CrossRefGoogle Scholar
  10. BRL, 1980, M13 cloning/dideoxy sequencing manual, Bethesda Research Laboratories, Inc. Brumbaugh, J. A., Middendorf, L. R., Grone, D. L., and Ruth, J. L., 1988, Proc. Natl. Acad. Sci. USA 85:5610–5614.PubMedCrossRefGoogle Scholar
  11. Church, G. M., and Gilbert, W., 1984, Proc. Natl. Acad. Sci. USA 81:1991–1995.PubMedCrossRefGoogle Scholar
  12. Church, G. M., and Kieffer-Higgins, S., 1988, Science 240:185–188.PubMedCrossRefGoogle Scholar
  13. Elder, J. K., Green, D. K., and Southern, E. M., 1986, Nucleic Acids Res. 14:417–424.PubMedCrossRefGoogle Scholar
  14. Frank, R., Bosserhoff, A., Boulin, C., Epstein, A., Gausepohl, H., and Ashman, K., 1988, BioTechnology 6:1211–1213.CrossRefGoogle Scholar
  15. Garoff, H., and Ansorge, W., 1981, Anal. Biochem. 115:450–457.PubMedCrossRefGoogle Scholar
  16. Hindley, J., 1983, DNA Sequencing, Elsevier, Amsterdam, pp. 200–206.Google Scholar
  17. Jablonski, E., Moomaw, E. W., Tullis, R. H., and Ruth, J. L., 1986, Nucleic Acids Res. 14(15):6115–6128.PubMedCrossRefGoogle Scholar
  18. Kambara, H., Nishikawa, T., Katayama, Y., and Yamaguchi, T., 1988, BioTechnology 6:816–821.CrossRefGoogle Scholar
  19. Landegren, U., Kaiser, R., Caskey, C. T., and Hood, L., 1988, Science 242:229–237.PubMedCrossRefGoogle Scholar
  20. Maxam, A. M., and Gilbert, W., 1977, Proc. Natl. Acad. Sci. USA 74:560–564.PubMedCrossRefGoogle Scholar
  21. Maxam, A. M., and Gilbert, W., 1980, Methods Enzymol. 65:499–560.PubMedCrossRefGoogle Scholar
  22. Messing, J., 1983, Methods Enzymol. 101:20–78.PubMedCrossRefGoogle Scholar
  23. Middendorf, L. R., Brumbaugh, J. A., Grone, D. L., Morgan, C. A., and Ruth, J. L., 1988, Am. Biotechnol. Lab. 6(6): 14–22.Google Scholar
  24. Mizusawa, S., Nishimura, S., and Seela, F., 1986, Nucleic Acids Res. 14(3): 1319–1324.PubMedCrossRefGoogle Scholar
  25. Nagai, K., Shimida, T., Tokita, J., Watanabe, K., Nakano, R., Kanbara, H., Sumitani, T., Teranishi, Y., and Hishinuma, F., 1987, Abstracts of the 1987 Pittsburgh Conference and Exposition on Analytical Chemistry and Applied Spectroscopy, Abstract 545 (March 9–13, 1987, Atlantic City, NJ).Google Scholar
  26. National Research Council, 1988, Mapping and Sequencing the Human Genome, National Academy Press, Washington, D.C.Google Scholar
  27. Olsson, A., Moks, T., Uhlen, M., and Gaal, A. B., 1984, J. Biochem. Biophys. Methods 10:83–91.PubMedCrossRefGoogle Scholar
  28. Page, G., 1988, Nature 333:477–478.CrossRefGoogle Scholar
  29. Pramik, M. J., 1988, Genetic Engineering News Jan. 1988, p. 6.Google Scholar
  30. Prentki, P., Karch, F., Iida, S., and Meyer, J., 1981, Gene 14:289–299.PubMedCrossRefGoogle Scholar
  31. Prober, J. M., Trainor, G. L., Dam, R. J., Hobbs, F. W., Robertson, C. W., Zagursky, R. J., Cocuzza, A. J., Jensen, M. A., and Baumeister, K., 1987, Science 238:336–341.PubMedCrossRefGoogle Scholar
  32. Ruth, J. L., 1984, DNA 3:123 (abstr.).Google Scholar
  33. Ruth, J. L., Morgan, C., and Pasko, A., 1985, DNA 4(1):93.Google Scholar
  34. Sanger, F., Niklen, S., and Coulson, A. R., 1977, Proc. Natl Acad. Sci. USA 74:5463–5467.PubMedCrossRefGoogle Scholar
  35. Smith, L. M., 1988, The Scientist July 25, 1988, p. 23.Google Scholar
  36. Smith, L. M., Sanders, J. Z., Kaiser, R. J., Hughes, P., Dodd, C., Connell, C. R., Heiner, C., Kent, B. H., and Hood, L., 1986, Nature 321:674–679.PubMedCrossRefGoogle Scholar
  37. Vieira, J., and Messing, J., 1987, Methods Enzymol 153:3–11.PubMedCrossRefGoogle Scholar
  38. Wada, A., 1984, Nature 307:193.CrossRefGoogle Scholar
  39. Wada, A., 1987, Nature 325:771–772.PubMedCrossRefGoogle Scholar
  40. West, J., 1988, Nucleic Acids Res. 16:1847–1856.PubMedCrossRefGoogle Scholar
  41. Wilson, R. K., Yuen, A. S., Clark, S. M., Spence, C., Arakelian, P., and Hood, L. E., 1988, BioTechniques 6:776–787.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • John Brumbaugh
    • 1
  • Lyle Middendorf
    • 2
  • Dan Grone
    • 2
  • Jerry Ruth
    • 3
  1. 1.School of Biological SciencesUniversity of NebraskaLincolnUSA
  2. 2.Li-Cor, Inc.LincolnUSA
  3. 3.Molecular Biosystems, Inc.San DiegoUSA

Personalised recommendations