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A Comparison of Lymphocyte Migration Across the Anterior and Posterior Blood-Retinal Barrier in Vitro

  • L. Devine
  • S. Lightman
  • J. Greenwood
Part of the Advances in Behavioral Biology book series (ABBI, volume 46)

Summary

The traffic of lymphocytes into the neuroretina of the eye is an important aspect of the development of immune mediated diseases of this part of the central nervous system (CNS). At the interface between the systemic immune system and the parenchyma lies a blood-tissue barrier which in the eye is referred to as the blood-retinal barrier. This barrier is situated at two separate sites, one being composed of the vascular endothelium and the other of retinal pigment epithelium (RPE). The differential migration of lymphocytes across these two cellular barriers and the factors controlling this process are just beginning to be understood. We have shown that T cell line lymphocytes are capable of migrating through monolayers of both vascular endothelial and RPE monolayers in vitro. The principle adhesion molecules involved in this process being the LFA-1/ICAM-1 receptor-ligand pairing with VLA-4/VCAM-1 playing a role following cytokine activation of the monolayers.

Keywords

Retinal Pigment Epithelium Experimental Autoimmune Encephalomyelitis Retinal Pigment Epithelium Cell Experimental Allergic Encephalomyelitis Purify Protein Derivative 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Résumé

Le trafic des lymphocytes dans la neurorétine de l’oeil est un aspect important du développement des maladies du système nerveux central (CNS) impliquant le système immunitaire. A l’interface entre le système immunitaire (circulation sanguine systémique) et le parenchyme se trouve une barrière sang-tissu qui au niveau de l’oeil porte le nom de barrière hémato-rétinienne. Cette barrière sépare l’endothélium vasculaire, d’une part et l’epithélium pigmenté de la rétine, d’autre part. Les différences de migration des lymphocytes à travers ces deux barrières cellulaires, ainsi que des facteurs contrôlant ce processus commencent tout juste à être étudiés. In vitro, nous avons montré que des lignées lymphocytaires T sont capables de migrer à travers les monocouches de l’endothélium vasculaire et de l’epithélium pigmenté de la rétine. Les principales molécules d’adhésion impliquées dans ce processus au niveau de ces monocouches sont les complexes récepteur-ligand LFAI/ICAM-1 et VLA-4/VCAM-1 après activation cytokines dépendante.

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References

  1. Calder, V.L. and Lightman, S.L. (1992). Experimental autoimmune uveoretinitis (EAU) versus experimental allergic encephalomyelitis (EAE): a comparison ofT cell-mediated mechanisms. Clin. Exp. Immunol. 89: 165–169.PubMedCrossRefGoogle Scholar
  2. Calder, V.L. and Greenwood, J. (1995). Role of the vascular endothelium in immunologically mediated neurological disease. In: Immunological aspects of the vascular endothelium. Savage, O.S. and Pearson, J.D. (eds) Cambridge University Press, Cambridge. pp. 96–123.CrossRefGoogle Scholar
  3. Caspi, R.R. (1989). Basic mechanisms in immune mediated uveitic disease. In: Immunology of Eye Diseases. Lightman, S. (ed.), Kluwer Academic Publishers, Dordrecht. pp. 61–87.CrossRefGoogle Scholar
  4. Chan, C., Palestine, A., Kuwabara, T. and Nussenblatt, R.B. (1988). Immunopathologic study of Vogt-Koyangai-Harada Syndrome. Am. J. Ophthalmol. 105: 607–611.PubMedGoogle Scholar
  5. Chang, C.W., Roque, R.S., Defoe, D.M. and Caldwell. R.B. (1991). An improved method for isolation and culture of pigmented epithelial cells from rat retina. Curr. Eye Res. 11: 1081–1086.CrossRefGoogle Scholar
  6. Kozak, Y., Sakai, J., Thillaye, B. and Faure, J.P. (1981). S-antigen-induced experimental autoimmune uveoretinitis in rats. Curr. Eye Res. 1: 327–337.PubMedCrossRefGoogle Scholar
  7. Devine, L., Lightman, S.L. and Greenwood, J. (1996). Role of LFA-1, ICAM-1, VLA-4 and VCAM-1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro. Immunology (submitted).Google Scholar
  8. Devine, L., Lightman, S.L. and Greenwood, J. (1996). Lymphocyte migration across the anterior and posterior blood-retinal barrier in vitro. Cell. immunol. (submitted).Google Scholar
  9. Dua, H.S., McKinnon, A., McMenamin, P.G. and Forrester, J.V. (1991). Ultrastructural pathology of the “barrier sites” in experimental autoimmune uveitis and experimental autoimmune pinealitis. Br. J. Ophthalmol. 75: 391–397.PubMedCrossRefGoogle Scholar
  10. Einer, S.G., Einer, V.M., Pavilack, M.A., Todd, R.F., Mayo-Bond, L., Franklin, W.A., Strieter, R.M., Kunkel, S.L. and Huber, A.R. (1992). Modulation and function of intercellular adhesion molecule-1 (CD54) on human retinal pigment epithelial cells. Lab. invest. 66: 200–211.Google Scholar
  11. Forrester, J.V., Liversidge, J., Towler, H. and McMenamin, P.G. (1990). Comparison of clinical and experimental uveitis. Curr. Eye Res. 9: 75–84.PubMedCrossRefGoogle Scholar
  12. Greenwood, J. (1992). Characterization of a rat retinal endothelial cell culture and the expression of P-glycoprotein in brain and retinal endothelium in vitro. J. Neuroimmunol. 39: 123–132.PubMedCrossRefGoogle Scholar
  13. Greenwood, J. and Calder, V.L. (1993). Lymphocyte migration through cultured endothelial cell monolayers derived from the blood retinal barrier. Immunology 80: 401–406.PubMedGoogle Scholar
  14. Greenwood, J., Howes, R. and Lightman, S. (1994). The blood-retinal barrier in experimental autoimmune uveoretinitis. Lab. Invest. 70: 39–52.PubMedGoogle Scholar
  15. Greenwood, J., Wang, Y.F. and Calder, V.L. (1995). Lymphocyte adhesion and transendothelial migration in the CNS: the role of LFA-1, ICAM-1, VLA-4 and VCAM-1. Immunology 86: 408–415.PubMedGoogle Scholar
  16. Hickey, W.F., Hsu, B.L. and Kimura, H. (1991). T-lymphocyte entry into the central nervous system. J. Neurosci. Res. 28: 254–260.PubMedCrossRefGoogle Scholar
  17. Jakobiec, F.A., Marboe, C.C., Knowles, D.M., Iwamoto, T., Harrison, W., Cleary, S. and Coleman, D.J. (1983). Human sympathetic ophthalmia. An analysis of the inflammatory infiltrate by hybridoma-monoclonal antibodies, immunohistochemistry, and correlative electron microscopy. Ophthalmology 90: 76–95.PubMedGoogle Scholar
  18. Lightman, S. and Chan, C.C. (1990). Immune mechanisms in choroido-retinal inflammation in man. Eye 4: 345–353.PubMedCrossRefGoogle Scholar
  19. Lightman, S. and Greenwood, J. (1992). Effect of lymphocyte infiltration on the blood-retinal barrier in experimental autoimmune uveoretinitis. Clin. Exp. Immunol. 88: 473–477.PubMedCrossRefGoogle Scholar
  20. Lightman, S. and Towler, H. (1992). Immunopathology and altered immunity in posterior uveitis in man: a review. Curr. Eye Res. 11: 11–15.PubMedCrossRefGoogle Scholar
  21. Lin, W. Essner, E. and Shichi, H. (1991). Breakdown of the blood-retinal barrier in S-antigen-induced uveoretinitis in rats. Gracfe’s Arch. Clin. Exp. Ophthalmol. 229: 457–463.CrossRefGoogle Scholar
  22. Liversidge, J., Sewell, H.F., and Forrester, J.V. (1990). Interactions between lymphocytes and cells of the blood-retina barrier: mechanisms of T lymphocyte adhesion to human capillary endothelial cells and retinal pigment epithelial cells in vitro. Immunology 71: 390–396.PubMedGoogle Scholar
  23. Male, D., Pryce, G., Linke, A. and Rahman, J. (1992). Lymphocyte migration into the CNS modelled in vitro.. J. Neuroimmunol. 40: 167–172.PubMedCrossRefGoogle Scholar
  24. Male, D. and Pryce, G. (1989). Induction of la molecules on brain endothelium is related to susceptibility to experimental allergic encephalomyelitis. J. Neuroimmunol. 21, 87–90.PubMedCrossRefGoogle Scholar
  25. Mirshahi, M., de Kozak, Y., Gregerson, D.S., Stiemer, R., Boucheix, C. and Faure, J.P. (1990). Genetic susceptibility of rat strains to induction of EAU in relation with the immune response to a particular epitope of S-antigen. Ocular Immunology Today, Proceedings of the Fifth International Symposium on the Immunology and Immunotherapy of the eye. pp. 195–198.Google Scholar
  26. Theofilopoulos, A.N. (1995). The basis of autoimmunity: Part II Genetic predisposition. Immunol. Today 16: 150–159.Google Scholar
  27. Wang, Y.F., Calder, V.L., Greenwood, J. and Lightman, S.L. (1993). Lymphocyte adhesion to cultured endothelial cells of the blood-retinal barrier. J. Neuroimmunol. 48: 161–168.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • L. Devine
    • 1
  • S. Lightman
    • 1
  • J. Greenwood
    • 1
  1. 1.Department of Clinical Ophthalmology, Institute of OphthalmologyUniversity College LondonLondonUK

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