Effects of Histamine on the Acid Phosphatase Activity of Cultured Cerebral Endothelial Cells
Acid phosphatase (orthophosphoric monoester hydrolase, EC 188.8.131.52) is widely distributed in nature and has been studied thoroughly in both plants and animals (Hollander, 1971). The enzyme is activated in acidic environment, which is provided by the presence of H+-ATPase, in different subcellular organelles of the exo- and endocytotic pathway (Mellman, Fuchs and Helenius, 1986). Consequently, the low pH provides favourable conditions for enzymatic hydrolyses in lysosomes; the proton gradient is used as an energy source for the coupled transport of biogenic amines. whereas the difference in pH between the endosome and the extracellular environment is used by the cell in receptor mediated endocytosis to provide symmetry to the recycling circuit between the two compartments (Mellman, Fuchs and Helenius, 1986).
KeywordsAcid Phosphatase Acid Phosphatase Activity Molecular Weight Form Cerebral Endothelial Cell APase Activity
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- Hollander, V.P. (1971) P.D. Boyer (ed): The enzymes. New York: Academic, pp. 449–498.Google Scholar
- Nosalova, V. and J. Navarova (1994) lndomethacine induced changes in mucosal lysosomal enzyme activity: effect of H2 antagonists. Agents Actions 41: C95 - C96.Google Scholar
- Roux, F., O. Durieu-Trautmann, N. Chaverot, M. Claire, P. Manly, J.M. Bourre, A.D. Strosberg, and P.-O. Couraud (1994) Regulation of gamma-glutamyl transpeptidase and alkaline phosphatase activities in immortalized rat brain microvessel endothelial cells. J. Cell Physiol. 159: 101–113.PubMedCrossRefGoogle Scholar
- Shimohama, S., S.Fujimoto (1993) Reduction of low-molecular-weight acid phosphatase activity in Alzheimer brains. Ann. Neurol. 33: 616–621.Google Scholar