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Biology and Physiology of the Blood-Brain Barrier pp 97–101Cite as

Membrane Fractionation of Brain Capillary Endothelial Cells and Analysis of Lipid Polarity

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Part of the book series: Advances in Behavioral Biology ((ABBI,volume 46))

Summary

Membrane fractionation of cultured porcine brain capillary endothelial cells (BCEC) was performed and the phospholipid composition of the obtained membrane fractions was analyzed. Strong differences between the apical and the basolateral membrane domains were observed for phosphatidylcholine pointing out that membrane lipids are polar distributed in BCEC.

Résumé

Nous avons étudié la composition en phospholipides des membranes de cultures de cellules endothéliales de microvaisseaux cérébraux de porc, après fractionnement membranaire.Nous avons observé que la composition des membranes apicales et basolatérales en phosphatidylcholine était différente, suggérant que la distribution membranaire des lipides est polarisée dans les cellules endothéliales de capillaires cérébraux de porc établies en culture.

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References

  1. Vorbrodt A.J.: Ultrastructural Cytochemistry of Blood-Brain Barrier Endothelia. G. Fischer Verlag, Stuttgart, New York (1988)

    Google Scholar 

  2. Simons, K. and van Meer, G.,Biochemistry 27, (1988) 6197–6202

    Article  CAS  Google Scholar 

  3. Grebenkemper, K and Galla, H.J. Chem. Phys. of Lip. 71, (1994) 133–143

    Article  Google Scholar 

  4. Stolz, D.B. and Jacobsen, B..J. Cell Sci. 103, (1992) 39–51

    CAS  Google Scholar 

  5. Mischek, U., Meyer, J. and Galla, H. J. Cell Tiss. Res. 256, (1989) 221–226

    Google Scholar 

  6. Macala, L.J., Yu, R.K. and Ando, J. Lip. Res. 24, (1983) 1243–1250

    CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Muenster, D-48149, Muenster, Germany

    Bernhard J. Tewes & Hans-Joachim Galla

Authors
  1. Bernhard J. Tewes
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  2. Hans-Joachim Galla
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Editor information

Editors and Affiliations

  1. Institut Cochin de Génétique Moléculaire, Paris, France

    Pierre-Olivier Couraud

  2. CNRS-Rhône-Poulenc Rorer, Vitry sur Seine, France

    Daniel Scherman

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© 1996 Springer Science+Business Media New York

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Tewes, B.J., Galla, HJ. (1996). Membrane Fractionation of Brain Capillary Endothelial Cells and Analysis of Lipid Polarity. In: Couraud, PO., Scherman, D. (eds) Biology and Physiology of the Blood-Brain Barrier. Advances in Behavioral Biology, vol 46. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9489-2_17

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  • DOI: https://doi.org/10.1007/978-1-4757-9489-2_17

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9491-5

  • Online ISBN: 978-1-4757-9489-2

  • eBook Packages: Springer Book Archive

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