How Does Thrombin Cause Neurite Retraction ?
Recent studies have established that thrombin modulates neuronal and glial cytoarchitecture. Thrombin antagonizes the neurite promoting activity of its inhibitor glia-derived nexin (Monard et al., 1983) and induces neurite retraction in mouse NB2a and human SK-N-SH neuroblastoma cells (Gurwitz and Cunningham, 1988; Grand et al., 1989), cultured human cerebellar and hippocampal neurons, and in AD12 HER10 cells of neuroepithelial origin (Grand et al., 1989; Grabham et al., 1991). Mouse dorsal root ganglion neurons grow shorter neuntes in the presence of thrombin (Hawkins and Seeds, 1986) and neuntes turn away from a source of thrombin placed in the culture system (Hawkins and Seeds, 1989). Thrombin has also been reported to reverse the cyclic AMP-induced stellation of cultured astrocytes and glioma cells (Cavanaugh et al., 1990, Nelson and Siman, 1990; Tas and Koschel, 1990).
KeywordsIntegrin Adenine Fibrinogen Neuroblastoma Prothrombin
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- Letourneau, P.C., 1989, Nerve cell shape, in “Cell Shape: Determinants, Regulation, andGoogle Scholar
- Regulatory Role“ pp 247–289. Bronner, F. and Stein, W.D., eds. Academic Press. New York. Lewis, S.D., Lorand, L., Fenton II, J.W. and Shafer, J.A., 1987, Catalytic competence of human sand y-thrombin in the activation of fibrinogen and factor XIII. Biochemistry 26:7597.Google Scholar
- Meyer, T., Regenass, U., Fabbro, D., Alteri, E., Rösel, J., Miller, M. Caravatti, G. and Matter, A., 1989, A derivative of staurosporine (CGP 41 251) shows selectivity for protein kinase C inhibition and in vitro anti-proliferative as well as in vivo anti-tumor activity. Int. J. Cancer 43: 851.Google Scholar
- Patterson, P.H., 1985, On the role of proteases, their inhibitors and the extracellular matrix in promoting neurite outgrowth. J. Physiol., Paris 80: 207.Google Scholar
- Rasmussen, U.B., Vouret-Craviari, V., Jallat, S., Schlesinger, Y., Pagès, G., Pavirani, A., Lecocq, J.-P., Pouysségur, J. and Van Obberghen-Schilling, E., 1991, cDNA cloning and expression of hamster a-thrombin receptor coupled to Cat+ mobilization. FEBS Lett. 288: 123.Google Scholar
- Rydel, R. E. and Green L.A., 1988, cAMP analogs promote survival and neurite outgrowth in cultures of rat sympathetic and sensory neurons independently of nerve growth factor. Proc. Natl. Acad. Sci. USA 85: 1257.Google Scholar
- Rydel, T.J., Ravichandran, K.G., Tulinsky, A., Bode, W., Huber, R., Roitsch, C. and Fenton II, J.F., 1990, The structure of a complex of recombinant hirudin and human a-thrombin. Science 249: 277.Google Scholar
- Vu, T.-K.H., Hung, D.T.,Wheaton, V.I., and Coughlin, S.R., 1991a, Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. Cell 64: 1057.Google Scholar
- Vu, T.-K.H., Wheaton, V.I., Hung, D.T., Charo, I., and Coughlin, S.R., 199 lb, Domains specifying thrombin-receptor interaction. Nature 353: 674.Google Scholar