Dynein-Microtubule Interactions

  • Nicolas J. Cordova
  • Ronald D. Vale
  • George F. Oster
Part of the NATO ASI Series book series (NSSB, volume 263)

Abstract

Vale and his colleagues (1989) devised a method of attaching single dynein molecules to a substrate and observing the motion of microtubules. In one series of experiments they inhibited the enzymatic activity of the dynein with vanadate. By fitting a Gaussian to this data, they obtained the diffusion constants shown in Table 1. However, according to the theoretical formulas for longitudinal and transverse diffusion of a cylinder these diffusion constants should be much larger. (The longitudinal and transverse diffusion coefficients are given by the following expressions: D L = kTln(2h/r)/2πηL and D T = kTln(2h/r)/4πηL (Brennen and Winet, 1977)). This discrepancy suggests two models for the interaction.

Keywords

Vanadate Librium Abate Peaked Vale 

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References

  1. 1.
    Feynman, R. P., Leighton, R. B., Sands, M. (1963). The Feynman Lectures on Physics. Addison-Wesley, Reading, Mass., pp. 46-1-46-5.Google Scholar
  2. 2.
    Goldstein, B., Delisi, C., Abate, J. (1975). Immunodiffusion in gels containing erythrocyte antigen. I. Theory for diffusion of antiserum from a circular well. J. Theor. Biol. 52, 317–334.CrossRefGoogle Scholar
  3. 3.
    Vale, R. D., Soll, D. M., Gibbons, I. R. (1989). One dimensional diffusion of microtubules bound to flagellar dynein. Cell. 59, 915–925.CrossRefGoogle Scholar
  4. 4.
    Vale, R. D. and Oosawa, F. (1990). Protein motors and Maxwell’s demons: does mechanochemical transduction involve a thermal ratchet? Adv. Biophys. 26, 97–134.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Nicolas J. Cordova
    • 1
  • Ronald D. Vale
    • 2
  • George F. Oster
    • 3
  1. 1.Department of Applied MathematicsWeizmann Institute of ScienceRehovotIsrael
  2. 2.Department of PharmacologyUniversity of California School of MedicineSan FranciscoUSA
  3. 3.Departments of Molecular and Cell Biology, and EntomologyUniversity of CaliforniaBerkeleyUSA

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