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Ice Strains of PseudomonasSyringae Introduced to Control Ice Nucleation Active Strains on Potato

  • Steven E. Lindow
Part of the NATO ASI Series book series (NSSA, volume 230)

Abstract

A common approach used in the biological control of plant diseases has been to introduce a single strain of a biological control agent to antagonize a target population. This “magic bullet” must antagonize all or most members of a target population to be effective in disease control (Spurr and Knudsen, 1985). Such an approach, however, generally assumes that the target microorganisms have similar ecological properties and display similar epidemiological features. For example, all members of a target population might be assumed to be sensitive to an antibiotic produced by a biological control organism or to utilize similar resources for which competition might be oc-curing. Molecular genetic tachniques have now demonstrated, however, that many plant pathogenic taxa are genetically diverse (Denney et al., 1988). While Restriction Fragment Length Polymorphism (RFLP) analysis of chromosome structure can give an indication of genetic diversity, it does not directly indicate physiological differences between members of a given group. However, the large genetic diversity found in many species of pathogens is suggestive that there will be functional differences in behavior of target microorganisms (Denney, 1988). The study reported here was made in an attempt to define the specificity of interactions of introduced biological control agents with their targets. The specific example to be discussed will be that of bacteria introduced to control epiphytic ice nucleation active (Ice+) bacteria.

Keywords

Biological Control Leaf Surface Competitive Exclusion Xanthomonas Campestris Navel Orange 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature Cited

  1. Burke, M. J., Gusta, L. A., Quamme, H. A., Weiser, C. J., and Li, P. H., 1976, Freezing and Injury to plants, Annu. Rev. Plant Physiol., 27: 507.CrossRefGoogle Scholar
  2. Cook, R. J., and Baker, K. F., 1983, “The Nature and Practice of Biological Control”, The American Phytopathological Society Press, St. Paul, MN.Google Scholar
  3. Denny, T. P., Gilmour, M. N., and Selander, R. K., 1988, Genetic diversity and relationships of two pathovars of Pseudomonas syringae, J. Gen. Microbiol., 134: 1949.PubMedGoogle Scholar
  4. Denny, T. P., 1988, Phenotypic diversity in Pseudomonas syringae pv. tomato, J. Gen. Microbiol., 134: 1939.Google Scholar
  5. deWit, C. T., 1960, On competition, Verslagen van landbouwkundige onderzoekingen, 660: 1.Google Scholar
  6. Kim, H. K., Orser, C., Lindow, S. E., and Sands, D. C., 1987, Xanthomonas campestris pv. translucens strains active in ice nucleation, Plant Dis., 71: 994.CrossRefGoogle Scholar
  7. Lindow, S. E., 1982, Population dynamics of epiphytic ice nucleation active bacteria on frost sensitive plants and frost control by means of antagonistic bacteria, pages 395–416, in: “Plant Cold Hardiness”, P. H. Li,and A. Sakai, eds., Academic Press, New York.Google Scholar
  8. Lindow, S. E., 1983a, The role of bacterial ice nucleation in frost injury to plants, Annu. Rev. Phytopathol., 21: 363.CrossRefGoogle Scholar
  9. Lindow, S. E., 1983b, Methods of preventing frost injury caused by epiphytic ice nucleation active bacteria, Plant Dis., 67: 327.CrossRefGoogle Scholar
  10. Lindow, S. E., 1985a, Integrated control and role of antibiosis in biological control of fireblight and frost injury, pages 83–115, in: “Biological Control on the Phylloplane”, C. Windels,and S. E. Lindow, eds., American Phytopathological Society Press, St. Paul, MN.Google Scholar
  11. Lindow, S. E., 1985b, Strategies and practice of biological control of ice nucleation active bacteria on plants, pages 293–311, in: “Microbiology of the Phyllosphere”, N. Fokkema, ed., Cambridge University Press, London.Google Scholar
  12. Lindow, S. E., 1985c, Ecology of Pseudomonas syringae relevant to the field use of Ice-deletion mutants constructed in vitro for plant frost control, pages 23–25, in: “Engineering Organisms in the Environment: Scientiifc Issues”, D. Pramer, ed., Am. Soc. Microbiology, Washington, D.C.Google Scholar
  13. Lindow, S. E., 1987, Competitive exclusion of epiphytic bacteria by Ice-mutants of Pseudomonas syringae, Appl. Environ. Microbiol., 53: 2520.PubMedGoogle Scholar
  14. Lindow, S. E., 1988a, Lack of correlation of antibiosis in antagonism of ice nucleation active bacteria on leaf surfaces by non-ice nucleation active bacteria, Phytopathology, 78: 444.CrossRefGoogle Scholar
  15. Lindow, S. E., 1988b, Construction of isogenic Ice-strains of Pseudomonas syringae for evaluation of specificity of competition on leaf surfaces, pages 509–515, in: “Microbial Ecology”, F. Megusar,and M. Gantar, eds., Slovene Society for Microbiology, Ljubljana.Google Scholar
  16. Lindow, S. E., 1989, Use of genetically altered bacteria to achieve plant frost control, pages 85–110, in: “Biotechnology of Plant-Microbe Interactions”, J. Nakas,and C. Hagedorn, eds., McGraw-Hill Publishing Co., New York.Google Scholar
  17. Lindow, S. E., 1990, Design and results of field trials of Ice-recombinant Pseudomonas syringae strains, pages 61–69, in: “Risk Assessment in Agricultural Biotechnology: Proceedings of the International Conference”, J. Marois,and J. Bruhning, eds., University of California, Oakland, CA.Google Scholar
  18. Lindow, S. E., 1991, Tests of specificity of competition among Pseudomonas syringae strains on plants using recombinant Ice-strains and use of nucleation genes as probes of in situ transcriptional activity, pages 457–465, in: “Molecular Genetics of Plant-Microbe Interactions-1990”, M. Heinrich, ed., Kluwer Academic Publishers, London.Google Scholar
  19. Lindow, S. E., Arny, D. C., and Upper, C. D., 1978a, Distribution of ice nucleation active bacteria on plants in nature, Appl. Environ. Microbiol., 36: 831.PubMedGoogle Scholar
  20. Lindow, S. E., Arny, D. C., and Upper, C. D., 1978b, Erwinia herbicola: a bacterial ice nucleus active in increasing frost injury to corn, Phytopathology, 68: 523.CrossRefGoogle Scholar
  21. Lindow, S. E., Arny, D. C., Barchet, W. R., and Upper, C. D., 1978, The role of bacterial ice nuclei in frost injury to sensitive plants, pages 249–263, in: “Plant Cold Hardiness and Freezing Stress”, P. Li, ed., Academic Press, New York.Google Scholar
  22. Lindow, S. E., Arny, D. C., and Upper, C. D., 1982, Bacterial ice nucleation: a factor in frost injury to plants, Plant Physiol., 70: 1084.PubMedCrossRefGoogle Scholar
  23. Lindow, S. E., Arny, D. C., and Upper, C. D., 1983a, Biological control of frost injury I: An isolate of Erwinia herbicola antagonistic to ice nucleation-active bacteria, Phytopathology, 73: 1097.CrossRefGoogle Scholar
  24. Lindow, S. E., Arny, D. C., and Upper, C. D., 1983b, Biological control of frost injury II: Establishment and effects of an antagonistic Erwinia herbicola isolate on corn in the field, Phytopathology, 73: 1102.CrossRefGoogle Scholar
  25. Lindow, S. E., and Panopoulos, N. J., 1988, Field tests of recombinant IcePseudomonas syringae for biological frost control in potato, pages 121–138, in: “Proceedings of the First International Conference on Release of Genetically Engineered Microorganisms”, M. Sussman, C. H. Collins, and F. A. Skinner, eds., Academic Press, London.Google Scholar
  26. Orser, C. S., Staskawicz, B. J., Panopoulos, N. J., Dahlbeck, D., and Lindow, S. E., 1985, Cloning and expression of bacterial ice nucleation genes in Escherichia coli, J. Bacteriol., 164: 359.PubMedGoogle Scholar
  27. Spurr, H. W., and Knudsen, G. R., 1985, Biological control of leaf diseases with bacteria, pages 45–62, in: “Biological Control on the Phylloplane”, C. E. Windels,and S. E. Lindow, eds., American Phytopathological Press, St. Paul, MN.Google Scholar
  28. Tilman, D., 1982, “Resource competition and community structure”, Princeton University Press, Princeton, New Jersey.Google Scholar
  29. Tilman, D., 1986, Resources, competition and the dynamics of plant communities, pages 51–75, in: “Plant Ecology”, M. J. Crawley, ed., Blackwell Scientiifc, Oxford.Google Scholar
  30. Wilson, M., and Lindow, S. E., 1991, Evidence for partinioning of nutrient resources among bacterial epiphytes in the phyllosphere, Phytopathology, (in press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Steven E. Lindow
    • 1
  1. 1.Department of Plant PathologyUniversity of CaliforniaBerkeleyUSA

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