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Electrical Currents Induced by Ion Pumps on Black Lipid Membranes

  • Ernst Bamberg
  • Klaus Fendler
Chapter
Part of the Ettore Majorana International Science Series book series (EMISS, volume 51)

Abstract

Charge translocation in light-driven and ATP-driven ion-pumps has been studied on planar lipid membranes. The method consists of the adsorption of membrane fragments containing the ion-pump to a planar lipid film. As examples the light-driven pump Bacteriorhodopsin and the Na+K+-ATPase from pig kidney have been investigated. In a short circuit experiment the displacement and pump currents were detected via the capacitive coupling between the membrane fragments and the underlying planar film.

Keywords

Equivalent Circuit Laser Flash Membrane Fragment Pump Current Black Film 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    J.H. Kaplan, B.I.I.I. Forbush and J.F. Hoffman, Biochemistry, 17, 1929 (1978).CrossRefGoogle Scholar
  2. [2]
    J.A. McCray, L. Herbette, T. Kihara and D.R. Trentham, Proc. Natl. Acad. Sci. USA, 77, 7237 (1980).CrossRefGoogle Scholar
  3. [3]
    W. Stoeckenius and R.A. Bogomolni, Ann. Rev. Biochem., 52, 587 (1982).CrossRefGoogle Scholar
  4. [4]
    D. Oesterhelt and W. Stoeckenius, Nature (London), 233, 149 (1971).Google Scholar
  5. [5]
    W. Stoeckenius, R.H. Lozier and R.A. Bogomolni, Biochim. Biophys. Acta, 505, 215 (1979).CrossRefGoogle Scholar
  6. [6]
    L.A. Drachev, D. Kaulen and V.P. Skulachev, FEBS Lett., 87, 161 (1978).CrossRefGoogle Scholar
  7. [7]
    M.C. Blok, K.J. Hellingwerf and K. Van Dam, FEBS Lett., 76, 45 (1977).CrossRefGoogle Scholar
  8. [8]
    Z.N. Dancshazy and B. Karvaly, FEBS Lett., 72, 136 (1976).CrossRefGoogle Scholar
  9. [9]
    H.W. Trissl and M. Montal, Nature (London), 266, 655 (1977).CrossRefGoogle Scholar
  10. [10]
    F.T. Hong and M. Montal, Biophys. J., 25, 465 (1979).CrossRefGoogle Scholar
  11. [11]
    L. Keszthelyi and P. Ormos, FEBS Lett., 109, 189 (1980).CrossRefGoogle Scholar
  12. [12]
    T.R. Herrmann and G.W. Rayfield, J. Biophys., 21, 111 (1978).CrossRefGoogle Scholar
  13. [13]
    E. Bamberg, H.J. Apell, N.A. Dencher, W. Sperling, H. Stieve and P. Läuger, Biophys, Struct. Mechanism, 5, 277 (1979).CrossRefGoogle Scholar
  14. [14]
    R.H. Lozier, R.A. Bogomolni and W. Stoeckenius, J. Biophys., 15, 955 (1975).CrossRefGoogle Scholar
  15. [15]
    F.S. Cohen, M. Eisenberg and S. McLaughlin, J. Memhr, Biol., 37, 361 (1978).CrossRefGoogle Scholar
  16. [16]
    A. Fahr. P. Läuger and E. Bamberg, J. Memhr. Biol, 60, 51 (1981).CrossRefGoogle Scholar
  17. [17]
    P. de Weer, Fortschr. Zool., 33, 387 (1986).Google Scholar
  18. [18]
    P. de Weer, D.C. Gadsby and R.F. Rakowski, Annu. Rev. Physiol., 50, 225 (1988).CrossRefGoogle Scholar
  19. [19]
    A.V. Lafaire and W. Schwarz, J. Membr. Biol, 91, 43 (1986).CrossRefGoogle Scholar
  20. [20]
    J.B. Chapman, E.A. Johnson and J.M. Kootsey, J. Membr. Biol, 74, 139 (1983).CrossRefGoogle Scholar
  21. [21]
    J.A. Reynolds, E.A. Johnson and C. Tanford, Proc. Natl. Acad. Sci. USA, 82, 6869 (1985).CrossRefGoogle Scholar
  22. [22]
    P. Läuger and H.J. Apell, Biochim. Biophys, Acta, 945, 1 (1988).CrossRefGoogle Scholar
  23. [23]
    K. Fendler, E. Grell, M. Haubs and E. Bamberg, EMBO J., 4, 3079 (1985).Google Scholar
  24. [24]
    G. Nagel, K. Fendler, E. Grell and E. Bamberg, Biochim. Biophys. Acta, 901, 239 (1987).CrossRefGoogle Scholar
  25. [25]
    K. Fendler, E. Grell and E. Bamberg, FEBS Lett., 224, 83, (1987).CrossRefGoogle Scholar
  26. [26]
    R. Borlinghaus, H.J. Apell and P. Läuger, J. Membr, Biol, 97, 161 (1987).CrossRefGoogle Scholar
  27. [27]
    H.J. Apell, R. Borlinghaus and P. Läuger, J. Membr, Biol, 97, 179 (1987).CrossRefGoogle Scholar
  28. [28]
    B. Forbush III in The sodium pump, I.M. Glynn and C. Ellory (Editors), Comp, of Biologists Ltd, G.B (1985), p. 599Google Scholar
  29. [29]
    R. Goldshlegger, S.J.D. Karlish and A. Rephaeli, J. Physiol, 387, 331 (1987).Google Scholar
  30. [30]
    M. Nakao and D.C. Gadsby, Nature (London), 323, 628 (1986).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Ernst Bamberg
    • 1
  • Klaus Fendler
    • 1
  1. 1.Max-Planck Institut für BiophysikFrankfurt 70Germany

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