Advertisement

Immunochemical Methods for Aflatoxins

  • Mary W. Trucksess
  • Samuel W. Page
Part of the Biodeterioration Research book series (BIOR, volume 3)

Abstract

Drought in 1988 caused a high incidence of aflatoxin contamination in corn samples in nine states including TX, OK, IA, IN, IL, SD, MD, WI and MN (Wall Street Journal, 1988a). In Illinois, 27% of 52 corn samples contained more than 20 ng aflatoxins/g (Wall Street Journal, 1988b). On October 4, 1988, the U.S. Food and Drug Administration (FDA) established enforcement levels for aflatoxins in corn used in feed (Rothschild, 1988). The levels were 100, 200 and 300 ng/g depending on the animal consuming the feed containing the aflatoxin-contaminated corn. FDA enforcement action could also be supported when corn containing in excess of 20 ng total aflatoxins/g was destined for food used by humans, for feed used by immature animals and by dairy animals, or if its destination was not known.

Keywords

Collaborative Study Wall Street Journal Peanut Butter Aflatoxin Contamination Immunoaffinity Column 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Candlish, A.A.G., Haynes, C.A. and Stimson W.H. (1988). Detection and determination of aflatoxins using affinity chromatography. Intern. J. Food Sci. Technol., 23, 479–485.CrossRefGoogle Scholar
  2. Chu, F.S. (in press). Immunoassay for mycotoxins: Current state of the art, commercial and epidemiological applications. Vet. Human Toxicol.Google Scholar
  3. Groopman, J.D. and Donahue, K.F. (1988). Aflatoxin, a human carcinogen: Determination in foods and biological samples by monoclonal antibody affinity chromatography. J. Assoc. Off. Anal. Chem., 71, 861–867.Google Scholar
  4. Park, D.L., Miller, B.M., Hart, L.P., Yang, G., McVey, J., Page, S.W., Pestka, J., and Brown, L.H. (1989a). Enzyme-linked immunosorbent assay for screening aflatoxin B1 in cottonseed products and mixed feed: Collaborative study. J. Assoc. Off. Anal. Chem., 72, 326–332.Google Scholar
  5. Park, D.L., Miller, B.M., Nesheim, S., Trucksess, M.W., Vekich, A., Bidigare, B., McVey, J.L., and Brown, L.H. (1989b). Visual and semiquantitative spectrophotometric ELISA screening method for aflatoxin B1 in corn and peanut products: Follow-up collaborative study. J. Assoc. Off. Anal. Chem., 72, 638–643.Google Scholar
  6. Pestka, J.J. (1988). Enhanced surveillance of foodborne mycotoxins by immunochemical assay. J. Assoc. Off. Anal. Chem., 71, 1075–1081.Google Scholar
  7. Pohland, A.E., Trucksess, M.W., Thorpe, C., and Clower, M. (1989). Immunoassays in food safety applications: Developments and perspectives, 198th ACS National Meeting, Abstract AGRO 45.Google Scholar
  8. Rothschild, L., Jr. (ed) (1988). FDA Sets Range of Enforcement Levels for Aflatoxin in Feed. Fd. Chem. News. October 10, 3-6.Google Scholar
  9. Trucksess, M.W., Stack, M.E., Nesheim, S., Park, D.L., and Pohland, A.E. (1989). Enzyme-linked immunosorbent assay of aflatoxins B1, B2, and G1 in corn, cottonseed, peanuts, peanut butter, and poultry feed: Collaborative study. J. Assoc. Off. Anal. Chem., 72, 957–962.Google Scholar
  10. Trucksess, M.W., Young, K., Donahue, K.F., Morris, D.K., and Lewis, E. (in press, 1990a). Comparison of two immunochemical methods with thin-layer Chromatographic methods for determination of aflatoxins. J. Assoc. Off. Anal. Chem.Google Scholar
  11. Trucksess, M.W., Stack, M.E., Nesheim, S., Page, S.W., Albert, R.H., Hansen, T.J., and Donahue, K.F. (in press, 1990b). Immunoaffinity column coupled with solution fluorometry or liquid chromatography postcolumn derivatization for determination of aflatoxins in corn, peanuts and peanut butter: Collaborative study. J. Assoc. Off. Anal. Chem. Google Scholar
  12. Wall Street Journal, September 30, 1988a.Google Scholar
  13. Wall Street Journal, October 6, 1988b.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Mary W. Trucksess
    • 1
  • Samuel W. Page
    • 1
  1. 1.Division of Contaminants ChemistryFood and Drug AdministrationUSA

Personalised recommendations