Techniques for Measurement of Zinc in Biological Samples
This chapter provides a brief description of techniques employed to assay zinc in plasma and blood cell in my laboratory. Many clinical laboratories use inaccurate and imprecise methods for assay of zinc. Since the levels of zinc in plasma and blood cells are very low, the methods must be very accurate in order to derive important biological information from this assay.
KeywordsNormal Saline Atomic Absorption Spectrophotometry Nitric Acid Solution Zinc Metal Transfer Pipette
Unable to display preview. Download preview PDF.
- Hudson, L., and Hay, F. C., 1980. Practical Immunology, 2nd ed., Blackwell, Oxford, p. 29.Google Scholar
- Milne, D. B., Ralston, N. V. C., and Wallwork, J. C., 1985. Zinc content of cellular components of blood: Methods for cell separation and analysis evaluated, Clin. Chem. 31: Washington, D.C., 65.Google Scholar
- Nishi, Y., Hatano, S., Horina, N., Sakano, T., and Usui, T., 1981. Zinc concentration in leukocytes: Mononuclear cell, granulocytes, T-lymphocytes, non-T lymphocytes and monocytes, Hiroshima J. Med. Sci. 30: 65.Google Scholar
- Prasad, A. S., Oberleas, D., and Halsted, J. A., 1965. Determination of zinc in biological fluids by atomic absorption spectrophotometry in normal and cirrhotic subjects, J. Lab. Clin. Med. 66: 508.Google Scholar
- Wang, H., Prasad, A. S., and DuMouchelle, E. A., 1989. Zinc in platelets, lymphocytes, and granulocytes by flameless atomic absorption spectrophotometry, J. Micron ut r. Anal. 5: 181.Google Scholar
- Whitehouse, R. C., Prasad, A. S., Rabbani, P. I., and Cossack, Z. T., 1982. Zinc in plasma, neutrophils, lymphocytes and erythrocytes as determined by flameless atomic absorption spectrophotometry, Clin. Chem. 28: Washington, D.C., 475.Google Scholar