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Developing Methods for a Thromboxane Synthetase Inhibitor and a Prostacyclin Analogue in Biological Fluids: Problems and Some Solutions

  • M. V. Doig
  • R. A. Clare
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

PG’s and TX’s have a wide range of biological activities including stimulation of muscles, dilation of small arteries, lowering of blood pressure, inhibition of gastric secretions and platelet aggregation. They are also implicated in imflammatory reactions, kidney function and immune responses [1]. Several articles in this vol. and in Vol. 16 deal with the determination of PG’s and TX’s and investigation of their biosynthesis. To define and elucidate their role in disease states, various inhibitors and analogues have been synthesized. These likewise have to be quantified at low levels without interference from endogenous compounds. Our experiences in developing two such methods are now described. In the first, solid-phase extraction problems arose.

Keywords

Gastric Secretion Methyl Formate Prostacyclin Analogue Free Silanols Thromboxane Synthetase Inhibitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Salmon, J.A. & Flower, R.J. (1983) in Hormones in Blood, 3rd edn., Vol. 2 (Gray, C.H. & James, V.H.T., eds.), Academic Press, New York, pp. 237–319.Google Scholar
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    McDowall, R.D., Pearce, J.C. & Murkitt, G S. (1986) J. Pharm. Biomed. Anal. 4, 3–21.CrossRefGoogle Scholar
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    Vendrig, D.E.M.M., Holthuis, J.J.M., Erdelyi-Toth, V. & Hulshoff, A. (1987) J. Chromatog. 414, 91–100.Google Scholar
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    Deans, D.R. (1968) Chromatographia 1, 18–22.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • M. V. Doig
    • 1
  • R. A. Clare
    • 1
  1. 1.Analytical and Clinical Pharmacokinetic Services DepartmentWellcome Research LaboratoriesBeckenham, KentUK

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