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A System Approach to Immunoassay Using Enhanced Luminescence

  • M. R. Summers
  • K. Mashiter
  • J. C. Edwards
  • J. K. Martin
  • G. P. Davidson
  • J. Holian
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

There is increasing awareness of the advantages of non-radioactive labels for heterogeneous, high sensitivity immunoassays. One of the most common systems is the enzyme immunoassay with colorimetric or fluorometric determination. However, because of the steady increase in signal as the reaction progresses, the signal development time (usually 15–30 min) must be accurately controlled, and the reaction stopped by reagent addition at a specific time. Chemiluminescence reactions provide an alternative end-point for such assays.

Keywords

Chemiluminescence Reaction Tracer Molecule Dedicated System Fluorometric Determination Sector Mass Spectrometer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • M. R. Summers
    • 1
  • K. Mashiter
    • 1
  • J. C. Edwards
    • 1
  • J. K. Martin
    • 1
  • G. P. Davidson
    • 1
  • J. Holian
    • 1
  1. 1.Amersham International plcAmersham, Bucks.UK

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