19F NMR Studies of the Metabolism of Trifluoromethylaniline
There is now much interest in the application of NMR techniques to determine the metabolic fate and disposition of foreign compounds [1–4] (& J.K. Nicholson in Vols. 16 & 17, this series — Ed.). For fluorinated compounds 19F NMR can be used with particular advantage due to its large chemical shift range giving good specificity, high sensitivity (83% of 1H) and the absence of endogenous fluorinated compounds in body fluids. In theory, drug recovery studies can be achieved by quantifying the spectra using a known concentration of a standard, thus obviating the need for expensive synthesis of radiolabelled compounds. To illustrate this, we have now investigated the metabolism and urinary excretion of TFMA* and its acetanilide. TLC and GC-MS studies  had shown that TFMA is metabolized to its oxanilic acid and a hydroxy metabolite which is probably glucuronidated (Fig. 1).
KeywordsHydroxy Metabolite Excretion Profile Radiolabelled Compound Fresh Urine Sample Pharmaceutical Research Laboratory
free induction decay
Unable to display preview. Download preview PDF.