Proton NMR Studies on the Metabolism and Biochemical Effects of Hydrazine In Vivo

  • S. M. Sanins
  • J. A. Timbrell
  • C. Elcombe
  • J. K. Nicholson
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)


High-resolution 1H NMR spectroscopy is valuable in the bioanalysis of biological fluids (e.g. [1–6], and Vols. 16 & 17 in this series — J.K. Nicholson). Notably, it allows quantitative information on a wide range of endogenous and xenobiotic metabolites to be collected simultaneously. As NMR is non-selective in the detection of metabolites (all compounds present at >0.1 mM levels being detectable), it is particularly appropriate for the investigation of the metabolism of compounds with poorly understood metabolic profiles.


Aliphatic Region Field Frequency Lock Xenobiotic Metabolite Hydrazine Metabolite 3Central Toxicology Laboratory 



1H-shift correlated


free induction decay [cf. GC use of ‘FID’-Ed,]


1,4,5,6-tetrahydro-6-oxo-3-pyridazine carboxylic acid


trichloroacetic acid


sodium d4-(trimethylsilyl)propionate


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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • S. M. Sanins
    • 1
  • J. A. Timbrell
    • 2
  • C. Elcombe
    • 3
  • J. K. Nicholson
    • 1
  1. 1.Analytical Sciences Group, Deprtment of ChemistryBirkbeck College (University of London)LondonUK
  2. 2.Toxicology UnitSchool of Pharmacy (University of London)LondonUK
  3. 3.Central Toxicology LaboratoriesICI plcMacclesfield, CheshireUK

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