Abstract
High-resolution 1H NMR spectroscopy is valuable in the bioanalysis of biological fluids (e.g. [1–6], and Vols. 16 & 17 in this series — J.K. Nicholson). Notably, it allows quantitative information on a wide range of endogenous and xenobiotic metabolites to be collected simultaneously. As NMR is non-selective in the detection of metabolites (all compounds present at >0.1 mM levels being detectable), it is particularly appropriate for the investigation of the metabolism of compounds with poorly understood metabolic profiles.
Keywords
- Aliphatic Region
- Field Frequency Lock
- Xenobiotic Metabolite
- Hydrazine Metabolite
- 3Central Toxicology Laboratory
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Abbreviations
- COSY:
-
1H-shift correlated
- Fid:
-
free induction decay [cf. GC use of ‘FID’-Ed,]
- HOPC:
-
1,4,5,6-tetrahydro-6-oxo-3-pyridazine carboxylic acid
- TCA:
-
trichloroacetic acid
- TSP:
-
sodium d4-(trimethylsilyl)propionate
References
Nicholson, J.K., Timbrell, J.A., Bales, J.R. & Sadler, P.J. (1985) Mol. Pharmacol. 27, 634–643.
Everett, J.R., Jennings, K. & Woodnut, G. (1985) J. Pharm. Pharmacol. 37, 869–873.
Bales, J.R., Nicholson, J.K. & Sadler, P.J. (1986) Clin. Chem. 31, 757–762.
Coleman, M.D. & Norton, R.S. (1986) Xenobiotica 16, 69–77.
Tulip, K., Timbrell, J.A., Wilson, I.D., Troke, J. & Nicholson, J.K. (1986) Drug Met. Disp. 14, 746–749.
Mclaughlin, A.C., Takeda, H. & Chance, B. (1979) Proc. Nat. Acad. Sci. 76, 5445–5449.
Jue, T., Arias-Mendoza, F., Gonnella, N.C., Shulman, G.I. & Shulman, R.G. (1985) Proc. Nat. Acad. Sci. 82, 5246–5249.
Allars, H., Coleman, M.D. & Norton, D.S. (1985) Eur. J. Drug Met. Pharmacol. 10, 253–260.
Cohen, S.M. (1983) J. Biol. Chem. 258, 14294–14308.
Cohen, S.M. (1984) Fed. Proc. 43, 2657–2662.
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Sanins, S.M., Timbrell, J.A., Elcombe, C., Nicholson, J.K. (1988). Proton NMR Studies on the Metabolism and Biochemical Effects of Hydrazine In Vivo . In: Reid, E., Robinson, J.D., Wilson, I.D. (eds) Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular. Methodological Surveys in Biochemistry and Analysis, vol 18 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9424-3_53
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