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Proton NMR Studies on the Metabolism and Biochemical Effects of Hydrazine In Vivo

  • S. M. Sanins
  • J. A. Timbrell
  • C. Elcombe
  • J. K. Nicholson
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

High-resolution 1H NMR spectroscopy is valuable in the bioanalysis of biological fluids (e.g. [1–6], and Vols. 16 & 17 in this series — J.K. Nicholson). Notably, it allows quantitative information on a wide range of endogenous and xenobiotic metabolites to be collected simultaneously. As NMR is non-selective in the detection of metabolites (all compounds present at >0.1 mM levels being detectable), it is particularly appropriate for the investigation of the metabolism of compounds with poorly understood metabolic profiles.

Keywords

Aliphatic Region Field Frequency Lock Xenobiotic Metabolite Hydrazine Metabolite 3Central Toxicology Laboratory 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

COSY

1H-shift correlated

Fid

free induction decay [cf. GC use of ‘FID’-Ed,]

HOPC

1,4,5,6-tetrahydro-6-oxo-3-pyridazine carboxylic acid

TCA

trichloroacetic acid

TSP

sodium d4-(trimethylsilyl)propionate

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • S. M. Sanins
    • 1
  • J. A. Timbrell
    • 2
  • C. Elcombe
    • 3
  • J. K. Nicholson
    • 1
  1. 1.Analytical Sciences Group, Deprtment of ChemistryBirkbeck College (University of London)LondonUK
  2. 2.Toxicology UnitSchool of Pharmacy (University of London)LondonUK
  3. 3.Central Toxicology LaboratoriesICI plcMacclesfield, CheshireUK

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