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An Approach to Metabolite Sample Preparation for Mass Spectrometry to Aid the Spectroscopist

  • R. D. Brownsill
  • A. Gray
  • C. W. Vose
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

Mass spectrometry (MS) is widely applied to the identification of drug metabolites isolated from biological fluids. A wide range of techniques are available, e.g. GC-MS and LC-MS where the MS options include electron-impact (EI), chemical ionization (CI) and fast atom bombardment (FAB). Similarly, many methods are available for metabolite isolation and purification, e.g. liquid-liquid and liquid-solid phase extraction, TLC, HPLC and electrophoresis. Despite this wide range of options, we may frequently experience problems with metabolite identification. These may result in part from certain assumptions:
  • metabolite identification is possible on ‘anything’;

  • isolation/purification techniques yield pure samples;

  • metabolite identification can be ‘fitted in’ as an ‘also-ran’.

Keywords

Chemical Ionization Fast Atom Bombardment Mass Spectrometry Method Insufficient Sample Metabolite Identification 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • R. D. Brownsill
    • 1
    • 2
  • A. Gray
    • 1
    • 2
  • C. W. Vose
    • 1
  1. 1.G.D. Searle and Co.High Wycombe, BucksUK
  2. 2.Servier R & DFulmer, SloughUK

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