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Identification of a New Metabolite of Leukotriene B4 by RP-HPLC and GC-MS Analysis

  • Joachim Fauler
  • Karl-Heinz Marx
  • Volkhard Kaever
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

RP-HPLC with UV detection is the method of choice for rapid identification and quantification of LT’s*. The large number of structurally related compounds precludes separating all of them within one system. Therefore RP-HPLC systems have been developed to isolate particular groups of compounds such as peptidoLT’s or LTB4 and its isomers. A RP-HPLC system now described allows the separation of a new metabolite of LTB4 from LTB4 and its trans isomers.

Keywords

Trans Isomer Tetraenoic Acid Lysosomal Enzyme Release Cultivate Mesangium Cell Human Inflammatory Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

LT

leukotriene

RP

reversed phase

MS

massspectrometry

PFB

pentafluorobenzyl

TMS

trimethylsilyl

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Notable References

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    Kaever, V., Martin, M., Fauler, J., Marx, K-H. & Resch, K. (1987) Biochim. Biophys. Acta 922, 337–344.CrossRefGoogle Scholar
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    Powell, W.S. (1987) Biochem. Biophys. Res. Comm. 145, 991–998.CrossRefGoogle Scholar
  3. 3.
    Matthews, W.R., Bundy, G.L., Wynalds, M.A., Guido, D.M., Schneider, S.P. & Fitzpatrick, F.A. (1988) Anal. Chem. 60, 349–353.CrossRefGoogle Scholar
  4. 4.
    Westcott, J.Y., Stenmark, K.R. & Murphy, R.C. (1986) Prostaglandins 31, 227–237.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Joachim Fauler
    • 1
  • Karl-Heinz Marx
    • 1
  • Volkhard Kaever
    • 2
  1. 1.Divisions of Clinical Pharmacology, Molecular Pharmacology, Department of PharmacologyToxicology Medical SchoolHannover 61Germany
  2. 2.Molecular Pharmacology, Department of PharmacologyToxicology Medical SchoolHannover 61Germany

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