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The Structure Elucidation of Drug Metabolites Using Thermospray LC-MS and LC-MS-MS

  • T. J. A. Blake
  • I. G. Beattie
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

The isolation of individual metabolites is often laborious and time-consuming, yet in the past was mandatory if structural characterization was required. The advent of directly coupled liquid chromatography [HPLC]-mass spectrometry (LC-MS) has greatly facilitated structure determination of drug metabolites without the need for isolation. This article concerns the thermospray interface, which has proved to be very reliable, and the combined potential of LC-MS and LC-MS-MS as highly specific identification techniques, along with collisionally activated decomposition, as shown for temelastine.

Keywords

Drug Metabolite Faecal Extract Collisionally Activate Decomposition Authentic Reference Compound Combine Potential 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Blakley, C.R. & Vestal, M.L. (1983) Anal. Chem. 55, 750–754.CrossRefGoogle Scholar
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    Blake, T.J.A. (1987) J. Chromatog. 394, 171–181.CrossRefGoogle Scholar
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    Durant, G.J., Ganellin, C.R., Griffiths, R., Harvey, C.A., Ife, R.J. & Owen, D.A.A. (1984) Br. J. Pharmacol. 82, Suppl., 232P.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • T. J. A. Blake
    • 1
  • I. G. Beattie
    • 1
  1. 1.Department of Drug MetabolismSmith Kline & French Research Ltd.The Frythe, Welwyn, HertsUK

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