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An Assay for Idaverine in Plasma and Urine at Picogram Level

  • D. J. K. van der Stel
  • O. A. M. Brockhoff
  • H. de Bree
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

The method now described was developed to establish the kinetics of idavevine and its metabolite, N-desmethylidaverine, in human plasma in the sub-ng range. The method hinges on a thorough clean-up procedure, which incorporates a solid-liquid extraction of the analytes from plasma, followed by lipid removal by washing with a hydrocarbon solvent. A liquid-liquid extraction is performed to remove acidic plasma constituents. Subsequently NP-HPLC⊗ is performed to separate the analytes from remaining ballast material, with an i.s. pair which functions also as carriers for the analytes. Finally separation and detection are performed by using automated on-column capillary GC with NPD.

Keywords

Parent Drug Potassium Carbonate Blank Plasma Determination Limit HPLC Mobile Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

NP[-HPLC]

normal- (straight-)phase

NPD

nitrogenphosphorus detector (N-FID)

i.s.

internal standard. ‘Ether’ is diethyl ether

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • D. J. K. van der Stel
    • 1
  • O. A. M. Brockhoff
    • 1
  • H. de Bree
    • 1
  1. 1.Analytical Development DepartmentDuphar Research LaboratoriesWeespThe Netherlands

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