The Determination of Plasma Amiodarone and Desethylamiodarone by HPLC
Since amiodarone may have serious side-effects, it is important to ascertain its levels in plasma*. We have developed a method more robust than existing HPLC methods. The sample, with i.s.⊗ added, is solvent-extracted from 1 M pH 6.0 acetate buffer, these conditions being optimal for the drug and its desethyl metabolite. RP-HPLC with UV detection is then performed. This validated assay is rapid and needs only small samples of serum, which may be pre-stored at 4° or −20°; if plasma stored at −20° is used, it may need centrifugation after thawing.
KeywordsIsosorbide Dinitrate Extraction Solvent Volume Tetramethylammonium Chloride Measure Peak Height Therapeutic Concentration Range
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