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Analysis of Eicosanoids by GC-MS/MS

  • J. C. Frölich
  • K.-H. Marx
  • H. Bippi
  • J. Fauler
  • B. Rosenkranz
  • U. Förstermann
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

Analysis of eicosanoids in biological matrices is difficult. Their levels are very low, and they are rapidly metabolized to numerous structurally similar substances often found in much higher concentration. Hence past assays of insufficient specificity have given erroneous values. GC-MS, which is more specific than RIA, is effective for structure elucidation and assay of eicosanoids and metabolites, yet may be insufficiently selective at low eicosanoia concentrations. PGE2 and PGF of renal origin are present in human urine, as now shown also for PGD2 by GC-MS/MS of the methoxime-TMS* ether of its methyl ester.

Keywords

Normal Volunteer Urine Flow Sodium Depletion Renal Origin Insufficient Specificity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

NCI

negative-ion chemical ionization

PG

prostaglandin

TMS

trimethylsilyl

i.s.

internal standard

2H

deuterium

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • J. C. Frölich
    • 1
  • K.-H. Marx
    • 1
  • H. Bippi
    • 1
  • J. Fauler
    • 1
  • B. Rosenkranz
    • 1
  • U. Förstermann
    • 1
  1. 1.Department of Clinical PharmacologyHannover Medical SchoolHannover 61W. Germany (Germany)

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