The Analysis of ACE Inhibitors by Immunoassay
IA* is one of the many techniques that have been used for the analysis of ACE inhibitors in biological fluids. The variety of IA procedures that can be employed are illustrated by the enzyme assays and RIA’s for captopril, cilazapril, enalapril, ramipril and trandolapril. Nearly all of these compounds have been analyzed using different variations of the IA approach. In many cases the assays have been developed to measure concentrations of the active metabolite only, concentrations of the parent compound being determined following in vitro conversion to the metabolite. This article reviews the different IA approaches that have been adopted for analyzing ACE inhibitors and comments on their features of interest.
KeywordsParent Compound Lysine Analogue Pool Normal Plasma Lysine Derivative Succinimidyl Propionate
angiotensin converting enzyme
enzyme-linked immunosorbent assay
bovine/human serum albumin
4-(maleimidoethyl)cyclohexane carboxylic acid
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