Analysis of Angiotensin-Converting Enzyme Inhibitors in Biological Fluids
During the last 10 years of development of several ACE* inhibitors, diverse analytical methods including HPLC, GC, GC-MS, REI and RIA have been reported. The different analytical methods are reviewed here with respect to their principles, procedures, strengths and weaknesses, except for RIA (considered in the following article). HPLC detection approaches, some entailing appropriate pre-derivatization, include fluorescence, electrochemical and even UV. Whatever the approach, captopril is detectable down to ~10–20 ng/ml, and pentopril to ~50 ng/ml. For GC measurement the approach, depending on the analyze, may be ECD, FID or NPD. Initial reaction steps followed by GC-MS with SIM have also been used for captopril and its mixed disulphides and S-methyl metabolites in different biological fluids. The sensitivity limit ranges between 1 and 20 ng/ml depending on the clean-up method and the detector used.
KeywordsHippuric Acid Blank Plasma Prodrug Ester Mixed Disulphide Reactive Thiol Group
selected ion monitoring
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- 3.Hayashi, K., Miyamoto, M. & Kripalini, K.J. (1980) J. Chromatog. 338, 161–169.Google Scholar
- 7.Perrett, D., Rudge, S.R. & Drury, P.L. (1984) Bioehem. Soc. Trans. 12, 1059–1060.Google Scholar
- 8.Shimada, K., Tanaka, M., Nambara, T., Imai, Y., Abe, K. & Yoshinaga, K. (1982) J. Chromatog. 227, 445–451.Google Scholar
- 10.Jemal, M., Ivashkiv, E., Ribick, M. & Cohen, A.I. (1985) J. Chromatog. 345, 299–307.Google Scholar
- 15.Drummer, O.H., Jarrott, B. & Lousi, W.J. (1984) J. Chromatog. 305, 83–93.Google Scholar
- 17.Fyrquist, F., Tikkanen, I., Gronhagen-Riska, C, Hortling, L. & Hichens, M. (1984) Clin. Chem. 30, 696–700.Google Scholar
- 20.Rakhit, A. & Tipnis, V. (1984) Clin. Chem. 30, 1237–1239.Google Scholar
- 21.Tipnis, V. & Rakhit, A. (1985) J. Chromatog. 345, 396–401.Google Scholar
- 23.Reydel-Bax, P., Redalieu, E. & Rakhit, A. (1987) Clin. Chem. 33, 549–553.Google Scholar