The Assay of Diclofenac and Metabolites: A Review

  • P. H. Degen
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

Diclofenac sodium, o-(2,6-dichlorophenyl)aminophenylacetic acid sodium salt, is a well-established potent NSAID*, given in low doses (25–100 mg/day). In man, diclofenac is extensively metabolized mainly by oxidative pathways followed by glucuronide conjugation. A small percentage of the original drug is glucuronidated directly. As diclofenac has a high total clearance, the plasma levels are low, necessitating a specific and very sensitive assay for pharmacokinetic studies.

Keywords

Hydrolysis HPLC Toluene Amide Phenyl 

Abbreviation

NSAITD

non-steroidal anti-inflammatory drug

i.s.

internal standard

ECD

electron-capture detector (similarly NPD, FID)

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • P. H. Degen
    • 1
  1. 1.Research and Development, Pharmaceuticals DivisionCiba-Geigy Ltd.BasleSwitzerland

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