Immunoassay of Beclomethasone 17,21-Dipropionate and Metabolites

  • W. N. Jenner
  • D. J. Kirkham
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)


A radioimmunoassay (RIA) has been developed for determining beclomethasone 17,21-dipropionate (BDP) in human body fluids, after solvent extraction, at levels down to 0.1 (plasma) or 1 (urine) ng/ml. The 3-O-carboxymethyloxime derivative of BDP was coupled by a mixed-anhydride reaction to bovine serum albumin (BSA) for raising a rabbit antiserum, or to [125 I] iodohistamine to provide a radiolabel. Known BDP metabolites and endogenous steroids had negligible cross-reactivity.


Assay Tube Endogenous Steroid Spiked Plasma Mixed Anhydride Antiserum Dilution 


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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • W. N. Jenner
    • 1
  • D. J. Kirkham
    • 1
  1. 1.Department of Biochemical PharmacologyGlaxo Group Research Ltd.Ware, Herts.UK

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