Penicillin-Binding Proteins 1A and 3 in Streptococcus Pneumoniae: What are Essential PBP’s?
Penicillin-binding proteins (PBPs) exist in every penicillin-susceptible bacterial species. One of the primary steps in the mechanism of action of β-lactams that finally lead to growth inhibition, lysis and death of the cell, is the interaction of penicillin with PBPs, the target enzymes for this group of antibiotics. Penicillin, believed to structurally resemble the actual substrate of PBPs — the C-terminal D-Ala-D-Ala moiety of muropentapeptides — becomes covalently bound to a serine residue located in the active site of all PBPs. This penicilloyl-PBP complex is enzymatically inactive, and therefore correct assembly of the murein layer which is vital for cell growth can no longer be ensured (for review, see Ghuysen, 1991).
KeywordsStreptococcus Pneumoniae Laboratory Mutant Murein Layer Interspecies Recombinational Event Essential PBPs
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