Advertisement

The Role of the envM Genes of Escherichia coli and Salmonella typhimurium in Cell Membrane Biosynthesis

  • Friederike Turnowsky
  • Helmut Bergler
  • Elisabeth Ingolic
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 65)

Abstract

Inhibition of the envM gene product by incubation of the conditional envM mutant Escherichia coli JP1111 at the nonpermissive temperature or by diazaborine treatment leads to pleiotropic effects on cell membrane composition. One of the most pronounced effects is the inhibition of phospholipid biosynthesis. The changes in the membrane structure lead to cell death without apparent lysis of the cells. The characteristic morphological changes are the retraction of the cytoplasmic membrane which leaves empty zones mainly at the poles of the cells. Overproduction of the wild type EnvM protein in the envM mutant at the nonpermissive temperature initially complements the ts phenotype but finally causes cell lysis.

Keywords

Viable Count Luria Broth Nonpermissive Temperature Phospholipid Biosynthesis Characteristic Morphological Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Egan, A.F. and Russell, R.R.B. (1973) Conditional mutants affecting the cell envelope of Escherichia coli K12. Genet.Res.Camb. 21, 139–152.CrossRefGoogle Scholar
  2. Högenauer, G. and Woisetschläger, M. (1981) A diazaborine derivative inhibits lipopolysaccharide biosynthesis. Nature 293, 662–664.PubMedCrossRefGoogle Scholar
  3. Reynolds, E.S. (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212.PubMedCrossRefGoogle Scholar
  4. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) “Molecular Cloning, a Laboratory Manual,” 2nd edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor.Google Scholar
  5. Silhavy, T.J., Berman, M.L., and Enquist, L.W. (1984) “Experiments with Gene Fusions,” Cold Spring Harbor Laboratory Press, Cold Spring Harbor.Google Scholar
  6. Spurr, A.R. (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J.Ultrastruct.Research 26, 31–43.CrossRefGoogle Scholar
  7. Turnowsky, F., Fuchs, K., Jeschek, C, and Högenauer, G.( 1989) envM genes of Salmonella typhimurium and Escherichia coli, J.Bacteriol. 171, 6555–6565.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Friederike Turnowsky
    • 1
  • Helmut Bergler
    • 1
  • Elisabeth Ingolic
    • 2
  1. 1.Institut für MikrobiologieUniversitätsplatz 2GrazAustria
  2. 2.Zentrum für ElektronenmikroskopieGrazAustria

Personalised recommendations