Distribution of O-Acetylation in the Peptidoglycan from Proteus mirabilis 19
The detection of O-acetylated peptidoglycan has previously required the isolation and purification of peptidoglycan from the study microorganisms followed by the release (saponification) and quantitation of acetate. Since this methodology is laborious and in addition, the ester-linked acetyl moiety is highly labile, it is not surprising that the prevalence of O-acetylated peptidoglycan among the eubacteria is largely unknown. The only microorganisms confirmed to possess O-acetylated peptidoglycan include Lactobacillus fermentum, Moraxella glucidolytica, Neisseria gonorrhoeae, N. perflava, Proteus mirabilis, P. vulgaris, Pseudomonas alcaligenes, Staphylococcus aureus and Streptococcus faecalis (reviewed in Clarke and Dupont, 1992). Many of these microorganisms represent important human pathogens but a correlation, if any, between peptidoglycan O-acetylation and pathogenicity/ virulence has not been examined. Moreover, very little is known regarding both the physiological role and the mechanistic process of the modification (Clarke and Dupont, 1992).
KeywordsImmunoelectron Microscopy Neisseria Gonorrhoeae Proteus Mirabilis Immunogold Labelling Booster Immunization
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