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Conjugative Plasmids of Streptomyces

  • David A. Hopwood
  • Tobias Kieser

Abstract

Genetic exchange in Streptomyces was first revealed when prototrophic recombinants were recovered from mixed cultures of pairs of auxotrophic derivatives of several wild-type strains (1, 8, 25, 63, 65). A conjugative mechanism, rather than transformation or transduction, was invoked to account for gene exchange because no recombinants were detected without prolonged physical contact of the parental strains (indeed, a period of mixed growth was needed) and because the pattern of inheritance of groups of markers was consistent only with recombination of large segments of the parental genomes (25). Plasmids were first clearly implicated in this conjugative process in the most studied strain, Streptomyces coelicolor A3(2), when certain derivatives of the wild-type isolate were found to differ in their “fertility” properties—that is, in the frequency with which they generated chromosomal recombinants when mated with various other derivatives—and this ability was inherited “infectiously” (2, 28, 72). These experiments led to the genetic definition of two conjugative plasmids—SCP1 and SCP2—that were deduced to be present in an autonomous state in the wild-type A3(2) strain and to be lost, or in the case of SCP1 sometimes chromosomally integrated, in various of its derivatives. Plasmids responsible for “fertility” (or “chromosome mobilizing ability” [Cma]) (24) were also identified genetically in some other strains, including Streptomyces rimosus (18), Streptomyces lividans (29), Streptomyces erythreus (now called Saccharopolyspora erythrea) (15),Streptomyces venezuelae (17), and Streptomyces ambofaciens (66).

Keywords

Terminal Inverted Repeat Linear Plasmid Plasmid Transfer Mycobacterium Smegmatis Conjugative Plasmid 
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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • David A. Hopwood
    • 1
  • Tobias Kieser
    • 1
  1. 1.John Innes InstituteJohn Innes CentreNorwichEngland

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