Skip to main content
SpringerLink
Log in

Conjugative Plasmids of Streptomyces

  • Chapter
Bacterial Conjugation

Abstract

Genetic exchange in Streptomyces was first revealed when prototrophic recombinants were recovered from mixed cultures of pairs of auxotrophic derivatives of several wild-type strains (1, 8, 25, 63, 65). A conjugative mechanism, rather than transformation or transduction, was invoked to account for gene exchange because no recombinants were detected without prolonged physical contact of the parental strains (indeed, a period of mixed growth was needed) and because the pattern of inheritance of groups of markers was consistent only with recombination of large segments of the parental genomes (25). Plasmids were first clearly implicated in this conjugative process in the most studied strain, Streptomyces coelicolor A3(2), when certain derivatives of the wild-type isolate were found to differ in their “fertility” properties—that is, in the frequency with which they generated chromosomal recombinants when mated with various other derivatives—and this ability was inherited “infectiously” (2, 28, 72). These experiments led to the genetic definition of two conjugative plasmids—SCP1 and SCP2—that were deduced to be present in an autonomous state in the wild-type A3(2) strain and to be lost, or in the case of SCP1 sometimes chromosomally integrated, in various of its derivatives. Plasmids responsible for “fertility” (or “chromosome mobilizing ability” [Cma]) (24) were also identified genetically in some other strains, including Streptomyces rimosus (18), Streptomyces lividans (29), Streptomyces erythreus (now called Saccharopolyspora erythrea) (15),Streptomyces venezuelae (17), and Streptomyces ambofaciens (66).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alikhanian, S. I., and Mindlin, S. Z., 1957, Recombinations in Streptomyces rimosus, Nature 180: 1208–1209.

    Article  PubMed  CAS  Google Scholar 

  2. Bibb, M. J., and Hopwood, D. A., 1981, Genetic studies of the fertility plasmid SCP2 and its SCP2’ variants in Streptomyces coelicolor A3(2), J. Gen. Microbiol. 126: 427–442.

    Google Scholar 

  3. Bibb, M. J., Schottel, J. L., and Cohen, S. N., 1980, A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces, Nature 284: 526–531.

    Article  PubMed  CAS  Google Scholar 

  4. Bibb, M. J., Ward, J. M., Kieser, T., Cohen, S. N., and Hopwood, D. A., 1981, Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans, Mol. Gen. Genet. 184: 230–240.

    Google Scholar 

  5. Bibb, M. J., Findlay, P. R., and Johnson, M. W, 1984, The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences, Gene 30: 157–166.

    Article  PubMed  CAS  Google Scholar 

  6. Bierman, M., Logan, R., O’Brien, K., Seno, E. T., and Schoner, B. E., 1992, Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp., Gene 116: 43–49.

    Article  PubMed  CAS  Google Scholar 

  7. Boccard, F., Pernodet, J.-L., Friedmann, A., and Guérineau, M., 1988, Site-specific integration of plasmid pSAM2 in Streptomyces lividans and S. ambofaciens, Mol. Gen. Genet. 212: 432–439.

    Google Scholar 

  8. Boccard, F., Smokvina, T, Pernodet, J.-L., Friedmann, A., and Guerineau, M., 1989, Structural analysis of loci involved in pSAM2 site-specific integration in Streptomyces, Plasmid 21: 59–70.

    Article  PubMed  CAS  Google Scholar 

  9. Boccard, F., Smokvina, T., Pernodet, J.-L., Friedmann, A., and Guérineau, M., 1989, The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages, EMBO J. 8: 973–980.

    PubMed  CAS  Google Scholar 

  10. Braendle, D. H., and Szybalski, W, 1959, Heterokaryotic compatibility, metabolic cooperation, and genetic recombination in Streptomyces, Ann. NY Acad. Sci. 81: 824–851.

    Google Scholar 

  11. Brody, H., Greener, A., and Hill, C. W, 1985, Excision and reintegration of the Escherichia coli K12 chromosomal element e14, J. Bacteriol. 161: 1112–1117.

    PubMed  CAS  Google Scholar 

  12. Buttner, M. J., and Brown, N. L., 1987, Two promoters from the Streptomyces plasmid pÚ101 and their expression in Escherichia coli, Gene 51: 179–186.

    Article  PubMed  CAS  Google Scholar 

  13. Chater, K. F., and Bruton, C. J., 1985, Resistance, regulatory and production genes for the antibiotic methylenomycin are clustered, EMBO J. 4: 1893–1897.

    PubMed  CAS  Google Scholar 

  14. Chung, S.-T., 1982, Isolation and characterization of Streptomyces fradiae plasmids which are prophage of actinophage •SF1, Gene 17: 239–246.

    Article  PubMed  CAS  Google Scholar 

  15. Deng, Z., Kieser, T., and Hopwood, D A., 1987, Activity of a Streptomyces transcriptional terminator in Escherichia coli, Nucl. Acids Res. 15: 2665–2675.

    Google Scholar 

  16. Deng, Z., Kieser, T., and Hopwood, D. A., 1988, “Strong incompatibility” between derivatives of the Streptomyces multi-copy plasmid p1. 1101, Mol. Gen. Genet. 214: 286–294.

    Google Scholar 

  17. Dewitt, J. P., 1985, Evidence for a sex-factor in Streptomyces erythreus, J. Bacteriol. 164: 969–971.

    PubMed  CAS  Google Scholar 

  18. Di Guglielmo, R., Conzelmann, C., Flett, E, and Cullum, J., 1990, J. Cellular Biochem. Suppl. 14A, Abstract CC 402, p. 124.

    Google Scholar 

  19. Doull, J. L., Vats, S., Chaliciopoulos, M., Stuttard, C., Wong, K., and Vining, L. C., 1986, Conjugational fertility and location of chloramphenicol biosynthesis genes on the chromosomal linkage map of Streptomyces venezuelae, J. Gen. Microbiol. 132: 1327–1338.

    Google Scholar 

  20. Friend, E. J., Warren, M., and Hopwood, D. A., 1978, Genetic evidence for a plasmid controlling fertility in an industrial strain of Streptomyces rimosus, J. Gen. Microbiol. 106: 201–206.

    Google Scholar 

  21. Gormley, E. P., and Davies, J., 1991, Transfer of plasmid RSF1010 by conjugation from Escherichia coli to Streptomyces lividans and Mycobacterium smegmatis, J. Bacteriol. 173: 6705–6708.

    PubMed  CAS  Google Scholar 

  22. Grant, S. R., Lee, S. C., Kendall, K., and Cohen,. S. N., 1989, Identification and characterization of a locus inhibiting extrachromosomal maintenance of the Streptomyces plasmid SLP1, Mol. Gen. Genet. 217: 324–331.

    Google Scholar 

  23. Gruss, A., and Ehrlich, S. D., 1989, The family of highly interrelated single-stranded deoxyribonucleic acid plasmids, Microbiol. Rev. 53: 231–241.

    Google Scholar 

  24. Guijarro, J., Santamaria, R., Schauer, A., and Losick, R., 1988, Promoter determining the timing and spatial localization of transcription of a cloned Streptomyces coelicolor gene encoding a spore-associated polypeptide, J. Bacteriol. 170: 1895–1901.

    PubMed  CAS  Google Scholar 

  25. Hanafusa, T., and Kinashi, H., 1991, The structure of an integrated copy of the giant linear plasmid SCP1 in the chromosome of Streptomyces coelicolor 2612, Mol. Gen. Genet. 231: 363–368.

    Google Scholar 

  26. Hardisson, C., and Manzanal, M. B., 1976, Ultrastructural studies of sporulation in Streptomyces, J. Bacteriol. 127: 1443–1454.

    PubMed  CAS  Google Scholar 

  27. Hirochika, H., Nakamura, K., and Sakaguchi, K., 1984, A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs, EMBO J. 3: 761–766.

    PubMed  CAS  Google Scholar 

  28. Holloway, B. W., 1979, Plasmids that mobilize bacterial chromosome, Plasmid 2: 1–19.

    Article  PubMed  CAS  Google Scholar 

  29. Hopwood, D. A., 1959, Linkage and the mechanism of recombination in Streptomyces coelicolor, Ann. NY Acad. Sci. 81: 887–898.

    Google Scholar 

  30. Hopwood, D. A., and Wright, H. M., 1973, A plasmid of Streptomyces coelicolor carrying a chromosomal locus and its inter-specific transfer, J. Gen. Microbiol. 79: 331–342.

    Google Scholar 

  31. Hopwood, D. A., and Wright, H. M., 1976, Genetic studies on SCP1-prime strains of Streptomyces coelicolor A3(2), J. Gen. Microbiol. 95: 107–120.

    Google Scholar 

  32. Hopwood, D. A., Chater, K. F., Dowding, J. E., and Vivian, A., 1973, Advances in Streptomyces coelicolor genetics, Bacteriol. Rev. 37: 371–405.

    Google Scholar 

  33. Hopwood, D. A., Kieser, T, Wright, H. M., and Bibb, M. J., 1983, Plasmids, recombination and chromosome mapping in Streptomyces lividans 66, J. Gen. Microbiol. 129: 2257–2269.

    Google Scholar 

  34. Hopwood, D. A., Lydiate, D. J., Malpartida, F., and Wright, H. M., 1985, Conjugative plasmids in Streptomyces, in: Plasmids in Bacteria ( D. Helinski, S. N. Cohen, D. B. Clewell, D. A. Jackson, and A. Hollaender, eds.), Plenum Press, New York, pp. 615–634.

    Chapter  Google Scholar 

  35. Hopwood, D. A., Kieser, T., Lydiate, D. J., and Bibb, M. J., 1986, Streptomyces plasmids: their biology and use as cloning vectors, in: The Bacteria. A Treatise on Structure and Function, Vol. IX ( S. W. Queener and L. E. Day, eds.), Academic Press, Orlando, pp. 159–229.

    Google Scholar 

  36. Hütter, R., and Eckhardt, T., 1988, Genetic manipulation, in: Actinomycetes in Biotechnology ( M. Goodfellow, S. T Williams, and M. Mordarski, eds.), Academic Press, London, pp. 89–184.

    Chapter  Google Scholar 

  37. Kalkus, J., Reh, M., and Schlegel, H. G., 1990, Hydrogen autotrophy of Nocardia opaca strains is encoded by linear megaplasmids, J. Gen. Microbiol. 136: 1145–1151.

    Google Scholar 

  38. Kataoka, M., Seki, T., and Yoshida, T, 1991, Five genes involved in self-transmission of pSN22, a Streptomyces plasmid, J. Bacteriol. 173: 4220–4228.

    PubMed  CAS  Google Scholar 

  39. Kataoka, M., Seki, T., and Yoshida, T, 1991, Regulation and function of the Streptomyces plasmid pSN22, genes involved in pock-formation and inviability, J. Bacteriol. 173: 7975–7981.

    PubMed  CAS  Google Scholar 

  40. c. Kataoka, M., Kuno, N., Horiguchi, T., Seki, T, and Yoshida, T, 1993, Replication of a Streptomyces plasmid pSN22 through single-stranded intermediates, J. Bacteriol., in press.

    Google Scholar 

  41. Keen, C. L., Mendelovitz, S., Cohen, G., Aharonowitz, Y., and Roy, K. L., 1988, Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus, Mol. Gen. Genet. 212: 172–176.

    Google Scholar 

  42. Kelemen, G H., Financsek, I., and Jerai, M., 1989, Efficient transformation of Micromonospora purpurea with pÚ702 plasmid, J. Antibiot. 42: 325–328.

    Article  PubMed  CAS  Google Scholar 

  43. Kendall, K., and Cohen, S. N., 1987, Plasmid transfer in Streptomyces lividans: identification of a kil-kor system associated with the transfer region of pÚ101, J. Bacteriol. 169: 4177–4183.

    PubMed  CAS  Google Scholar 

  44. Kendall, K., and Cohen, S. N., 1988, Complete nucleotide sequence of the Streptomyces lividans plasmid pU101 and correlation of the sequence with genetic properties, J. Bacteriol. 170: 4634–4651.

    PubMed  CAS  Google Scholar 

  45. Kendall, K., and Cullum, J., 1984, Cloning and expression of an extracellular-agarase gene from Streptomyces coelicolor A3(2) in Streptomyces lividans 66, Gene 29: 315–321.

    Article  PubMed  CAS  Google Scholar 

  46. Kendall, K., and Cullum, J., 1986, Identification of a DNA sequence associated with plasmid integration in Streptomyces coelicolor A3(2), Mol. Gen. Genet. 202: 240–245.

    Google Scholar 

  47. Kieser, H. M., Henderson, D. J., Chen, C. W, and Hopwood, D. A., 1989, A mutation of Streptomyces lividans that prevents intraplasmid recombination has no effect on chromosomal recombination, Mol. Gen. Genet. 220:60—M.

    Google Scholar 

  48. Kieser, H. M., Kieser, T., and Hopwood, D. A., 1992, A combined genetic and physical map of the Streptomyces coelicolor A3(2) chromosome, J. Bacteriol. 174: 5496–5507.

    PubMed  CAS  Google Scholar 

  49. Kieser, T., Hopwood, D. A., Wright, H. M., and Thompson, C. J., 1982, pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors, Mol. Gen. Genet. 185: 223–238.

    Google Scholar 

  50. Kieser, T., and Hopwood, D. A., 1991, Genetic manipulation of Streptomyces: integrating vectors and gene replacement. Meth. in Enzymol. 204: 430–458.

    Google Scholar 

  51. Kinashi, H., and Shimaji-Murayama, M., 1991, Physical characterization of SCP1, a giant linear plasmid from Streptomyces coelicolor, J. Bacteriol. 173: 5123–1529.

    Google Scholar 

  52. Kinashi, H., Shimaji, M., and Sakai, A., 1987, Giant linear plasmids in Streptomyces which code for antibiotic biosynthesis genes, Nature 328: 454–456.

    Article  PubMed  CAS  Google Scholar 

  53. Kinashi, H., Shimaji-Murayama, M., and Hanafusa, T., 1991, Nucleotide sequence analysis of the unusually long terminal inverted repeats of a giant linear plasmid, SCP1, Plasmid 26: 123–130.

    Article  PubMed  CAS  Google Scholar 

  54. Kinashi, H., Shimaji-Murayama, M., and Hanafusa, T., 1992, Integration of SCP1, a giant linear plasmid, into the Streptomyces coelicolor chromosome. Gene 115: 35–41.

    Article  PubMed  CAS  Google Scholar 

  55. Kirby, R., and Hopwood, D. A., 1977, Genetic determination of methylenomycin synthesis by the SCP1 plasmid of Streptomyces coelicolor A3(2), J. Gen Microbiol 98: 239–252.

    Article  PubMed  CAS  Google Scholar 

  56. Kirby, R., Wright, L. F, and Hopwood, D. A., 1975, Plasmid-determined antibiotic synthesis and resistance in Streptomyces coelicolor, Nature 254: 265–267.

    Article  PubMed  CAS  Google Scholar 

  57. Kobayashi, T, Shimotsu, H., Horinouchi, S., Uozumi, T, and Beppu, T., 1984, Isolation and characterization of a pock-forming plasmid pTA4001 from Streptomyces lavendulae, J. Antibiot. 37: 368–375.

    Article  PubMed  CAS  Google Scholar 

  58. Kuhstoss, S., Richardson, M. A., and Rao, R. N., 1989, Site-specific integration in Streptomyces ambofaciens: localization of integration functions in S. ambofaciens plasmid pSAM2, J. Bacteriol. 171: 16–23.

    PubMed  CAS  Google Scholar 

  59. Lee, S. C., Orner, C. A., Brasch, M. A., and Cohen, S. N., 1988, Analysis of recombination occurring at SLP1 an sites, J. Bacteriol. 170: 5806–5813.

    PubMed  CAS  Google Scholar 

  60. Lee, M. H., Pascopella, L., Jacobs, W. R., and Hatfull, G. R., 1991, Site-specific integration of mycobacteriophage L5 integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis and Bacille Calmette-Guerin. Proc. Natl. Acad. Sci. USA 88: 3111–3115.

    Google Scholar 

  61. Martin, J. F., and Liras, P., 1989, Organization and expression of genes involved in the biosynthesis of antibiotics and other secondary metabolites, Annu. Rev. Microbiol. 43: 173–206.

    Google Scholar 

  62. Martin, C., Mazodier, P., Mediola, M. V., Gicquel, B., Smokvina, T., Thompson, C. J., and Davies, J., 1991, Site-specific integration of the Streptomyces plasmid pSAM2 in Mycobacterium smegmatis, Molec. Microbiol. 5: 2499–2502.

    Google Scholar 

  63. Matsushima, P., and Baltz, R. H., 1988, Genetic transformation of Micromonospora rosaria by the Streptomyces piasmid pÚ702, J. Antibiot. 41: 583–585.

    Article  PubMed  CAS  Google Scholar 

  64. Matsushima, P., McHenney, M. A., and Baltz, R. H., 1987, Efficient transformation of Amycolaropsis orientalis ( Nocardia orientalis) protoplasts by Streptomyces plasmids, J. Bacteriol. 169: 2298–2300.

    Google Scholar 

  65. Mazodier, P., Petter, R., and Thompson, C., 1989, Intergeneric conjugation between Escherichia coli and Streptomyces species, J. Bacteriol. 171: 3585–3585.

    Google Scholar 

  66. Mazodier, P., Thompson, C., and Boccard, F., 1990, The chromosomal integration site of the Streptomyces element pSAM2 overlaps a putative tRNA gene conserved among actinomycetes, Mol. Gen. Genet. 222: 431–434.

    Google Scholar 

  67. Nordstrom, K., and Austin, S. J., 1989, Mechanisms that contribute to the stable segregation of plasmids, Annu. Rev. Genet. 23: 37–69.

    Google Scholar 

  68. Orner, C. A., and Cohen S. N., 1984, Plasmid formation in Streptomyces: excision and integration of the SLP1 replicon at a specific chromosomal site, Mol. Gen. Genet. 196: 429–438.

    Google Scholar 

  69. Omer, C. A., and Cohen, S. N., 1986, Structural analysis of plasmid and chromosomal loci involved in sitespecific excision and integration of the SLP1 element of Streptomyces coelicolor, J. Bacteriol. 166:999–1006. 56a. Orner, C. A., Stein, D., and Cohen, S. N., 1988, Site-specific insertion of biologically functional adventitious genes into the Streptomyces lividans chromosome, J. Bacteriol. 170: 2174–2184.

    Google Scholar 

  70. Pernodet, J.-L., and Guerineau, M., 1981, Isolation and physical characterization of streptomycete plasmids, Mol. Gen. Genet. 182: 53–59.

    Google Scholar 

  71. Pidcock, K. A., Montenecourt, B. S., and Sands, J. A., 1985, Genetic recombination and transformation in protoplasts of Thermomonospora fusca, Appl. Environment. Microbiol. 50: 693–695.

    Google Scholar 

  72. Pierson, L. S., and Kahn, M. L., 1987, Integration of satellite bacteriophage P4 in Escherichia coli: DNA sequences of the phage and host regions involved in site-specific recombination, J. Mol. Biol. 196: 487–496.

    Google Scholar 

  73. Pigac, J., Vujaklija, C., Toman, Z., Gamulin, V, and Schrempf, H., 1988, Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces, Plasmid 19: 222–230.

    Article  PubMed  CAS  Google Scholar 

  74. Rafii, F., and Crawford, D. L., 1989, Donor/recipient interactions affecting plasmid transfer among Streptomyces species: a conjugative plasmid will mobilize nontransferable plasmids in soil, Curr. Microbiol. 19: 115–121.

    Google Scholar 

  75. Reiter, W-D., Palm, P., and Yeats, S., 1989, Transfer RNA genes frequently serve as integration sites for prokaryotic genetic elements, Nucl. Acids Res. 17: 1907–1914.

    Google Scholar 

  76. Saito, H., and Ikeda, Y., 1959, Cytogenetic studies on Streptomyces griseoflavus, Ann. NY Acad. Sci. 81: 862–878.

    Google Scholar 

  77. Sakaguchi, K., 1990, Invertrons, a class of structurally and functionally related genetic elements that includes linear DNA plasmids, transposable elements, and genomes of adeno-type viruses, Microbiol. Rev. 54: 66–74.

    Google Scholar 

  78. Sermonti, G., and Spada-Sermonti, I., 1955, Genetic recombination in Streptomyces, Nature (London) 176: 121.

    Article  CAS  Google Scholar 

  79. Shiffman, D., and Cohen, S. N., 1992, Reconstruction of a Streptomyces linear replicon from separately cloned DNA fragments—existence of a cryptic origin of circular replication within the linear plasmid, Proc. Natl. Acad. Sci. USA 89: 6129–6133.

    Google Scholar 

  80. Smokvina T., Francou, F, and Luzzati, M., 1988, Genetic analysis in Streptomyces ambofaciens, J. Gen. Microbiol. 134: 395–402.

    Google Scholar 

  81. Smokvina, T., Mazodier, P., Boccard, F, Thompson, C. J., and Guérineau, M., 1990, Construction of a series of pSAM2-based integrative vectors for use in actinomycetes, Gene 94: 53–59.

    Article  PubMed  CAS  Google Scholar 

  82. Smokvina, T., Boccard, F., Pernodet, J.-L., Friedmann, A., and Guérineau, M., 1991, functional analysis of the Streptomyces ambofaciens elements pSAM2, Plasmid 25: 40–52.

    Google Scholar 

  83. Stein, D. S. and Cohen, S. N., 1990, Mutational and functional analysis of the korA and korB gene products of Streptomyces plasmid pIJ101, Mol. Gen. Genet. 222: 337–344.

    Google Scholar 

  84. Stein, D. S., Kendall, K. J., and Cohen, S. N., 1989, Identification and analysis of transcriptional regulatory signals for the kil and kor loci of Streptomyces plasmid pU101, J. Bacteriol. 171: 5768–5775.

    PubMed  CAS  Google Scholar 

  85. Tsai, J. F.-Y., and Chen, C. W, 1989, Isolation and characterization of Streptomyces lividans mutants deficient in intraplasmid recombination, EMBO J. 8: 973–980.

    Google Scholar 

  86. Vivian, A., 1971, Genetic control of fertility in Streptomyces coelicolor A3(2): plasmid involvement in the interconversion of OF and IF strains, J. Gen. Microbiol. 69: 353–364.

    Google Scholar 

  87. Vivian, A., and Hopwood, D. A., 1973, Genetic control of fertility in Streptomyces coelicolor A3(2): new kinds of donor strains, J. Gen. Microbiol. 76: 147–162.

    Google Scholar 

  88. Wellington, E. M. H, Cresswell, N., and Saunders, V. A., 1990, Growth and survival of streptomycete inoculants and extent of plasmid transfer in sterile and nonsterile soil, Appl. Environment. Microbiol. 56: 1413–1419.

    Google Scholar 

  89. Wu, X., and Roy, K. L., 1993, Complete nucleotide sequence of a linear plasmid from Streptomyces clavuligerus, and characterization of its RNA transcripts, J. Bacteriol. 175: 37–52.

    PubMed  CAS  Google Scholar 

  90. Yamamoto, H., Maurer, K. H., and Hutchinson, C. R., 1986, Transformation of Streptomyces erythreus, J. Antibiot. 39: 1304–1313.

    Article  PubMed  CAS  Google Scholar 

  91. Zaman, S., Richards, H., and Ward, J., 1992, Expression and characterization of the korB gene product from the Streptomyces lividans plasmid pU101 in Escherichia coli and determination of its binding site on the korB and kilB promoters, Nucl. Acids Res. 20: 3693–3700.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. John Innes Institute, John Innes Centre, Norwich, NR4 7UH, England

    David A. Hopwood & Tobias Kieser

Authors
  1. David A. Hopwood
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tobias Kieser
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Biologic and Materials Sciences, School of Dentistry and Department of Microbiology and Immunology, School of Medicine, The University of Michigan, 48109-0402, Ann Arbor, Michigan, USA

    Don B. Clewell

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer Science+Business Media New York

About this chapter

Cite this chapter

Hopwood, D.A., Kieser, T. (1993). Conjugative Plasmids of Streptomyces . In: Clewell, D.B. (eds) Bacterial Conjugation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9357-4_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-9357-4_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-44376-3

  • Online ISBN: 978-1-4757-9357-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation