Secondary Metabolites as a Tool in Ascomycete Systematics: Lichenized Fungi

  • William Louis Culberson
  • Chicita F. Culberson
Part of the NATO ASI Series book series (NSSA, volume 269)


For many years, the secondary products called lichen substances have been intimately involved in the systematics of the lichen-forming ascomycetes. Today they take on a new importance as markers in genetic studies and for the analysis of gene flow and reproductive isolation. Lichen systematics has been much hampered in that artificial crosses are not possible. Refined methods of thin-layer chromatography and high-performance liquid chromatography now make it possible to chemotype single-spore cultures grown in vitro and consequently to analyze the progeny of maternal individuals from nature. Since lichen fungi are haploid, the appearance of a progeny chemotype different from the maternal one can detect and identify the source of gene flow. Examples from the Cladonia chlorophaea and Ramalina siliquosa species complexes are used to demonstrate this approach to the analysis of gene exchange in lichen fungi.


Gene Flow Reproductive Isolation Hybrid Zone Usnic Acid Southern Appalachian Mountain 
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  1. Ahmadjian, V., and J.B. Jacobs, 1981, Relationship between fungus and alga in the lichen Cladonia cristatella Tuck., Nature 289: 169–172.CrossRefGoogle Scholar
  2. Caisse, M., and J. Antonovics, 1978, Evolution in closely adjacent plant populations. IX. Evolution of reproductive isolation in clinal populations, Heredity 40: 371–384.CrossRefGoogle Scholar
  3. Culberson, CF., 1965, Some constituents of the lichen Ramalina siliquosa, Phytochemistry 4: 951–961.CrossRefGoogle Scholar
  4. Culberson, C.F., W.L. Culberson, and D.A. Arwood, 1977, Physiography and fumarprotocetraric acid production in the Cladonia chlorophaea group in North Carolina, Bryologist 80: 71–75.CrossRefGoogle Scholar
  5. Culberson, C.F., W.L. Culberson, and A. Johnson, 1988, Gene flow in lichens, American Journal of Botany 75: 1135–1139.CrossRefGoogle Scholar
  6. Culberson, C.F., W.L. Culberson, and A. Johnson, 1992, Characteristic lichen products in cultures of chemotypes of the Ramalina siliquosa complex, Mycologia 84: 705–714.CrossRefGoogle Scholar
  7. Culberson, C.F., W.L. Culberson, and A. Johnson, 1993, Occurrence and histological distribution of usnic acid in the Ramalina siliquosa species complex, Bryologist 96: 181–184.CrossRefGoogle Scholar
  8. Culberson, W.L., 1967, Analysis of chemical and morphological variation in the Ramalina siliquosa species complex, Brittonia 19: 333–352.CrossRefGoogle Scholar
  9. Culberson, W.L., 1986, Chemistry and sibling speciation in the lichen-forming fungi: ecological and biological considerations, Bryologist 89: 123–131.CrossRefGoogle Scholar
  10. Culberson, W.L., and C.F. Culberson, 1967, Habitat selection by chemically differentiated races of lichens, Science 158: 1195–1197.PubMedCrossRefGoogle Scholar
  11. Culberson, W.L., C.F. Culberson, and A. Johnson, 1977, Correlations between secondary-product chemistry and ecogeography in the Ramalina siliquosa group (lichens), Plant Systematics and Evolution 127: 191–200.CrossRefGoogle Scholar
  12. Culberson, W.L., C.F. Culberson, and A. Johnson, 1993, Speciation in the lichens of the Ramalina siliquosa complex (Ascomycotina, Ramalinaceae): gene flow and reproductive isolation, American Journal of Botany 80: 1472–1481.CrossRefGoogle Scholar
  13. DePriest, P.T., and M.D. Been, 1992, Numerous Group I introns with variable distributions in the ribosomal DNA of a lichen fungus, Journal of Molecular Biology 228: 315–321.PubMedCrossRefGoogle Scholar
  14. Dickinson, H., and J. Antonovics, 1973, Theoretical considerations of sympatric divergence, American Naturalist 107: 256–274.CrossRefGoogle Scholar
  15. Ehrlich, P.R., and P.H. Raven, 1969, Differentiation of populations, Science 165: 1228–1232.PubMedCrossRefGoogle Scholar
  16. Felsenstein, J., 1981, Skepticism towards Santa Rosalia, or why are so there so few kinds of animals?, Evolution 35: 124–138.CrossRefGoogle Scholar
  17. Harrison, R.G., 1990, Hybrid zones: windows on evolutionary process, In: Oxford Surveys in Evolutionary Biology (D. Futuyma and J. Antonovics, eds) 7: 69–128. Oxford University Press, New York.Google Scholar
  18. Hewitt, G.M., 1989, The subdivision of species by hybrid zones, In: Speciation and its Consequences (D. Otte and J. A. Endler, eds): 85–110. Sinauer Associates, Sunderland.Google Scholar
  19. Levene, H., 1953, Genetic equilibrium when more than one ecological niche is available, American Naturalist 87: 331–333.CrossRefGoogle Scholar
  20. Levin, D.A., and H.W. Kerster, 1967, Gene flow in seed plants, Evolutionary Biology 1: 139–220.Google Scholar
  21. Lewis, J.R., 1964, The Ecology of Rocky Shores, English Universities Press, London.Google Scholar
  22. Mattsson, J.-E., and J. Kärnefelt, 1986, Protein banding patterns in the Ramalina siliquosa group, Lichenologist 18: 231–240.CrossRefGoogle Scholar
  23. Maynard Smith, J., 1966, Sympatric speciation, American Naturalist 100: 637–650.CrossRefGoogle Scholar
  24. Purvis, O.W., B.J. Coppins, D.L. Hawksworth, P.W. James, and D.M. Moore, 1992, The Lichen Flora of Great Britain and Ireland, Natural History Museum Publications, London.Google Scholar
  25. Sheard, J.W., 1978a, The taxonomy of the Ramalina siliquosa species aggregate (lichenized ascomycetes), Canadian Journal of Botany 56: 916–938.CrossRefGoogle Scholar
  26. Sheard, J.W., 1978b, The comparative ecology and distribution and within-species variation in the lichenized ascomycetes Ramalina cuspidata and R. siliquosa in the British Isles, Canadian Journal of Botany 56: 939–952.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • William Louis Culberson
    • 1
  • Chicita F. Culberson
    • 1
  1. 1.Department of BotanyDuke UniversityDurhamUSA

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